Ermin Rachmawati, Risma Aprinda Kristanti, Nurlaili Susanti


Background: Several epidemiological data’s reported significant correlation between fructose consumption and diabetes mellitus type 2 by inducing insulin resistance. Leptin resistance induced by fructose was proposed as one novel mechanism that induce insulin resistance but the exact mechanism remains unclear.  We hypothesize that fructose diminish the type b leptin receptors in hypothalamus and intestine.

Aim: This study was aimed to elucidate fructose effect on the expression of leptin receptor type b in hypothalamus and intestine of Rattus novergicus.

Method: twenty eight rats were used and divided into 4 groups: Group 1 was control, group 2 was given fructose 10%, group 3 was given fructose 30% and group 4 was given fructose 55% for 2 months. At the end of treatment, the animal were sacrificed and then the hypothalamus and intestine were collected. The expression of type b leptin receptor were measured by immunohistochemistry technique with primary antibody from Bioss antibodies type Leptin receptor polyclonal antibody bs-0109R using Staining kit Skytec Laboratories and DAB chromogen. A positive expression can be seen as a brown colour in cell cytoplasm and counted in 100 cell. The expression then analysed using SPSS 18 with anova one way tes (p<0,05) followed by post hoc test after the data showed normality and homogeneity using Saphiro wilk and Levene test (p>0,05).

Result: There was significant differences in type b leptin receptors found in hypothalamus between each group (p<0.05). The significant differences also could be seen in the expression between control and group fructose 30 and 50% in intestinal cell (p<0.05).

Conclusion: the consumption of Fructose 55% for 2 months attenuates the expression of type b leptin receptors in hypothalamus and intestine of Rat novergicus.


Full Text:



Akar F, Uludag O, Aydin A, Aytekin YA, Elbeg S, Tuzcu M, Sahin K. 2012. High fructose corn syrup causes vascular dysfunction associated with metabolic disturbances in rats : Protective effect of resveratrol." Food and chemical Toxicology 50: 2135-2141.

Bezzera RMN, Ueno M, Silva MS, Tavares DQ, Carvalho CRO etal.2001.A high fructose diet induces insulin resistance but nod blood pressure changes in normotensive rats. Braz J Med Biol Res 34(9):1155-60

Bursac BN, Vasiljević AD, Nestorović NM, Veličković NA, Vojnović Milutinović DD etal.2014.High fructose diets leads to visceral adiposity and hypothalamic leptin resistance in male rats. J Nutr Biochem 25(4):446-55

Elliot SS, Keim NL, Stern JS, Teff K dan Havel PJ.2002.Fructose, weight gan and the insulin resistance. Am J Clin Nutr 76:911-22

French SA, Lin B, Guthrie JF. 2003.National trends in soft drink consumption among children and adolescents age 6 to 17 years : Prevalence, amounts and sources 1977/1978 to 1994/1998.J Am Diet Assoc 103:1326-1331.

Harring SJ dan Harris RBS.2011.The relation between dietary fructose, dietary fat and leptin responsiveness in rats. Physiol Behav 104(5):914-22

Malik, Popkin BM, Bray, GA, Despres J-P, Hu FB. 2010. Sugar Sweetened Beverages, Obesity, Type 2 Diabetes and. Circulation 12 (11): 1356-1364.

Martinez FJ, Rizza RA, Romero JC. 1994. High fructose feeding elicit insulin resistance, hyperinsulinemia, and hypertension in normal mongrel dogs. Hypertension 23: 456-463.

Morris DL, Cho KW, Zhou Y, Rui L. SH2B1 enhances insulin sensitivity by both stimulating the insulin receptor and inhibiting tyrosine dephosphorylation of insulin receptor substrate proteins.Diabetes. 2009; 58(9):2039–2047. [PubMed: 19542202]

Nielsen SJ and Popkin BM.2004.Changes in Beverage intake between 1977 and 2001.Am J Prev Med 27(3)205-210

Putnam JJ, Allshouse JE. 1999. Food consumption, prices, and expenditures, 1970–97. Washington, DC: Economic Research Service, US Department of Agriculture, Roglans N, Vila L, Farre M, Alegret M, Sanchez RM, Vazquez-Carrera M, Laguna C.2007. Impairment of hepatic Stat-3 activation and redux=ction of PPAR activity in male rats. Hepatology 45:778-788

Shapiro A, Mu E, Roncal C, Cheng KY, Johnson RJ, Scarpace PJ.2008.Fructose-induced leptin resistance exacerbates weight gain in response to subsequent high fat feeding

Souza AM, Pereira RA, Yokoo EM, Levy RB, Sichieri R.2013.Most consumed foods in Brazil:National Dietary Survey 2008-2009.Rev Saude Publica 47.

Walker RW, Dumke KA, Goran MI. 2014. Fructose content in popular beverages made with and without high fructose corn syrup.Nutrition 30: 928-935.



  • There are currently no refbacks.

Editorial Office:

Faculty of Medical and Health Sciences,
Universitas Islam Negeri Maulana Malik Ibrahim
Jalan Ir. Soekarno No.34 Dadaprejo, Batu, Jawa Timur, Indonesia
Phone (+62) 85746163772, (+62) 85259506000, Faximile (+62) 341 558933

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.