Uji Efektifitas Daya Hambat Sabun Cair Cuci Tangan di Kota Surabaya Terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherechia Coli secara in Vitro

Ira Purbosari

Abstract


Currently, the rise of the corona disease (Covid-19) has become a worldwide pandemic which has been declared by Who to be the center of attention around the world. One of the prevention efforts is to wear a mask and wash hands. Hand washing is done by using liquid soap and rinsing with soapy water. The degree of acidity (pH) of soap serves to inhibit the growth rate and kill bacteria. This research is to see the antibacterial activity of unbranded and branded liquid soap products and have distribution permits that are traded freely in the marketplace. Using the disc diffusion technique method, the zone of inhibition is determined. In this study, a positive control was used, namely liquid soap that already had a distribution permit and a negative control in the form of aquadest. The test results obtained that the diameter of the inhibition zone for liquid soap, namely S. aureus in liquid soap, was 27.7 mm and in positive control was 24.9 mm. Meanwhile, in E.coli, the average diameter of the inhibition area in liquid soap was 29.3 mm and in positive control was 32.8 mm. This is influenced by the concentration of antiseptic, soap composition, antibacterial, and pH of the soap, as well as bacterial cell wall components and dilution. And from the results of statistical analysis using the Tukey test method, it was found that there was a significant difference in liquid soap (p-value 0.000) which was significant in inhibiting the rate of bacterial growth.

Keywords: bacterial growth, escherichia coli, hand washing, inhibition test, liquid soap, staphylococcus aureus


References


World Health organization (WHO), (2020). Corona Virus (Covid-19). New York

Kampf et al, 2020.Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents.Journal of Hospital Infection 104(2020) 246-251

Xia ma et al, 2020.Potentiaence of coronal utilities of mask wearing and instant hand hygiene for fighting SARS-CoV-2. Journal of Medical Virology 2020;1-5

Burton et al, 2011.The Effet of Handwashing with Water or Soap on Bacterial Contamination of Hands.Int.J.environ.Res.Public Health 2011, 8,97-104

Loho, T.,Utami,L.,effectivity Test of Antiseptic Solution 17o Triclosan against Satphylococcum aureus, Escherichia coli, enterococcus faecalis, and Pseudomonas aeruginosa, majalah Kedokteran Indonesia, 57(6), 175-178, departemen Patologi Klinik Fakultas Kedokteran Universitas Indonesia, Jakarta.2007

Who, 2009. WHO Guidelines on hand Hygiene in Health care

Widmer,2000. Replace hand washing with Use of a Waterless Alcohol hand Rub?. Clinical Infectious Disease 2000;31;136-43

Mwambete KD, Lyombe F.Antimicrobial activity of Medicated soaps commonly used by dar es salaam resicents in Tanzania.Indian Journal of Pharmaceutical sciences.2011;7391):92-8

Selvamohan T, Sandhya, V. Studies on bactericidal strains. Journal of Microbiology and Biotechnology Research.2012;2(5):646-50.

Greenwood, 2000. Detection of antibiotic resistance in vitro. International Journal of Antimicrobial agents 14(4):303-6

Brooks GF, Butel JS, Morse SA.jawetz Melnick, & adelberg’s medical microbiology. Edisi ke-25. United State of America: The McGraw-Hill Companies, Inc;2010.

Putra IA.Uji Efek Antibakteri Ekstrak etanol Kulita batang Salam (syxygium polyanthum weight walp) terhadap staphylococcus aureus dan eschereccia coli secara in vitro. Padang: Universitaa andalas; 2013.

Aliyu MS, Tijjani MB, doko MHI, Garba I, Ibrahim MM, Abdulkadir SM, et al. Antimicrobial activity of sabulun solo a local tradisional medicated soap.Nigerian Journal of Basic and Applied Science.2012;20(1): 35-8

Oranusi US, Akanade VA, dahunsi SO.Assesment of microbial quality and antibacterial activity of commonly used hand washes. Journal of Biological and Chemical Research.2013;30(2):570-8




DOI: https://doi.org/10.18860/jip.v6i1.12146

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