DETERMINATION OF THIAMINE HYDROCHLORIDE AND PYRIDOXINE HYDROCHLORIDE ON RICE WITH WASHING, MILLING AND POUNDING BY SPECTROMETRIC METHODS

Edy Agustian Yazid, Damar Pasunaring Ati, Aulia Mahardika

Abstract


Rice is a principal food source for most of the world's population. Rice has many nutrients including thiamin hydrocloride (THC) and pyrodoxine hydrocloride (PHC). Before consumption, rice is usually processed such as milling, washing, and cooking. Determination of THC on IR-64 and Rojolele rice with washing three times has been conducted. Also determined of PHC on the milled IR-64 rice and the pounded IR-64 rice. The THC was determined by spectrophotometric method at 246 nm. Determination of PHC based on the coupling reaction with diazo reagent in alkaline solution to form azo dye then the absorption was measured at 440 nm. The result of determination of THC on IR-64 and Rojolele rice after washing three times can decrease THC level 12,93% and 12,88% respectively. Determination of PHC on the milled IR-64 rice obtained 1.54 ± 0.027 mg/g and on the pounded IR-64 rice 1.80 ± 0.048 mg/g. The result of statistical test with independent T test showed there were significant difference (α = 0,05) PHC level on both treatments.

Full Text:

PDF

References


Schwartz, S.J. 1991. World Rise Trade: Prospects and Issue for the Nineties. Rice Situation and Outlook Report, USDA Economic Research Service.

Widjayanti, E. 2004. Potential and Prospect of Indigenous Indonesian Functional Food. National Food Colloquium, Bandung.

Tien, R.M., Sugiyono, Fitriyono, A. 2010. Knowledge of Food. Alfabeta, Bogor: pp. 277-282.

Indrasari S.D, Wibowo P, and Ardhiyanti S.D. 2011. Evaluation of Nutritional Quality and Acidity of Folic Acid Several Red Rice Chaffs. Proceedings of Indonesian Center for Rice Research, Bogor: pp.1293-1300.

Putra, S. 2010. Exploration and Characterization of Red Rice Germplasm Plasma in Solok Regency of West Sumatra. Thesis Undergraduate Degree, Faculty of Agriculture Andalas University, Padang.

Made, O.A. 2001. Application of the Nerlove Partial Adjustment Model in Production Projection and Rice Consumption. Center for Food Crops R & D, Bogor.

Gealy, David R and Bryant RJ. 2009. Seed Physicochemical Characteristics of Field-grown US Weedy Rice (Oryza sativa) Biotypes: Contrasts with Commercial Cultivars. Journal of Cereal Science, 49:239-245.

Almatsier S. 2009. Basic Principles of Nutrition Science. Gramedia Pustaka Utama, Jakarta: pp. 213-220

Norouzi, T., Garakani, M., Rashedi, H.A. Zamani, M.R., Ganjali. 2010. Ultrasensitive Flow-Injection Electrochemical Method Using Fast Fourier Transform Square-Wave Voltammetry for Detection of Vitamin B1. International Journal of Electrochemistry Science, 5: 639 – 652.

Lynch P.L, Young I.S. 2000. Determination Of Thiamine by High Performance Liquid Chromatography. Journal of Chromatography, 881:267–84.

Bhagwat G.B. 2013. UV-Spectrophotometric Analysis of Prochlorperazine Maleate and Pyridoxine in Tablet Dosage Form. International Journal of Chemistry, 5(5):2309-16.

Murray, R.K., Granner, D.K., Mayes, P.A., Rodwell, V.W. 1997. Harper Biochemistry. 24th Ed, Medical Book Publishers EGC, Jakarta.

Thomson, A.D. 2000. Mechanisms of Vitamin Deficiency in Chronic Alcohol Misusers and The Development of The Wernicke-Korsakoff Syndrome. International Journal of Pharmacy and Pharmaceutical Sciences, 35(1):2–7.

Niraimathi, V., Jerad, S.A, Nanjappan, K. 2010. Spectrophotometric Estimation of Nislodipine in Bulk and Tablet Dosage Formulation. Acta Ciencia Indica, 36(4): 393.

Tjiptadi, W. 1982. Rice and Processing. Faculty of Agricultural Technology, IPB Bogor.

Andarwulan, N and Koswara, SK. 1992. Chemical Vitamins, Rajawali Press, Jakarta: pp. 215-220.

Novi T.N., Widowati, D., Wartini., Aryani, T. 2011. Validation of HPLC Method for Determination of Thiamine Hydrochloride, Riboflavin, Nicotinamide, and Pyridoxine Hydrochloride in Syrup Preparation. Canadian Journal on Scientific and Industrial Research, 7: 269-274.

Rohman, A. 2007. Chemical Pharmaceutical Analysis. Pustaka Pelajar. Yogyakarta: pp.157-160.

Manohar, SD, Sridhar, D.A, Allikarjuna, S.C. 2013. Development of UV Spectrophotometric Method for Estimation of Carvedilol in Bulk and Pharmaceutical Formulations. Asian Journal of Research Chemistry, 10:111-118.

Pathak, A., Rajput, S. (2008). Simultaneous Determination Of Ternary Mixture Of Pyridoxine Hydrochloride, Doxylamine Succinate and Folic Acid by a Ratio Spectra Zero Crossing, Double Divisor-Ratio Spectra Derivative and Column HPLC Methods. Journal of AOAC International, 91(5):1059-1069.

Raza, A., Ansari. T., Niazi, S., Rehman, A. 2007. Spectrophotometric Determination of Pyridoxine Hydrochloride (Vitamin B6) in Bulk and Tablets by Charge-Transfer Complexation with Chloranil. Journal of Chemical Society of Pakistan, 29(1):33-36.

Smita, C.N, Preeti, V., Kulkarni., Bhaskar, V., Chavhan, V. 2013. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Doxylamine Succinate and Pyridoxine Hydrochloride in Bulk and Tablet Dosage Form, International Journal of Pharmacy and Pharmaceutical Sciences, 5(3):390-393.

Kaynak. M. S. and Sahin. S.A. 2008. New HPLC Approach for Determination of In-Vitro Solubility of Naproxen Sodium Hacettepe. University Journal of the Faculty of Pharmacy, 28(1):49-62.

Sudarmadji, Haryono, B., and Suhardi. 1997. Analytical Procedures for Food and Agricultural Materials. Liberty,Yogyakarta.

Auterhoff dan Kovar. 2002. Identify of Drugs. ITB, Bandung: pp. 169-170.

Harmita. 2004. Implementation Instructions Validation Method and Method Calculation. Pharmaceutical Science, Magazine of Indonesia University, 3:117-134.

Sediaoetama, A.D. 2004. Nutrition Science, 5th Ed, Dian Rakyat, Jakarta

Villareal, C and B. Juliano. 1989. Variability in contents of thiamin and riboflavin in brown rice, crude oil in brown rice and bran-polish, and silicon in hull of IR rices. Plants Foods for Human Nutrition, 39:287-297.

Kennedy, G and Burlingame, B. 2003. Analysis Of Food Composition Data on Rice From A Plant Genetic Resources Perspective. Journal Food Chemistry, 80:589-596.




DOI: http://dx.doi.org/10.18860/jip.v3i2.6136

Refbacks

  • There are currently no refbacks.


© 2019 Journal of Islamic Pharmacy - All rights reserved