EFFECT OF COLLAGEN CONCENTRATION ON MORPHOLOGY OF PVA/CHITOSAN FIBERS MADE BY ELECTROSPINNING METHOD

Ni Nyoman Rupiasih, Dewa Ayu Sukma Pranastia, Made Sumadiyasa, P. B. Vidyasagar

Abstract


The purpose of this study was to determine the impact of the addition of collagen concentration on morphology of PVA/chitosan nanofiber. The research was conducted by making 10% PVA solution, 2% chitosan solution, and collagen solutions with various concentrations of 2%, 4%, and 8%. The PVA/chitosan/collagen nanofibers were made with ratios of 8:2:2, 8:2:4, and 8:2:8 and were named P8/Ch2/C2, P8/Ch2/C4, and P8/Ch2/C8. As a control was PVA/chitosan with a ratio of 8:2, which was named P8/Ch2/C0. The nanofiber was made by electrospinning method with the following parameters: a voltage of 10 kV, a distance from the spinneret tip to the collector of 10 cm, and a flow rate of 1.00 µl/h. Nanofiber characterization was carried out using FTIR and SEM. SEM images were analyzed using ImageJ and Origin to measure the diameter and length of the fibers. It obtained that, the best ratio of PVA/chitosan/collagen to make a good nanofiber is 8:2:2, with the characteristics are the least number of beads and solution droplets, the most continuous fiber, and the average diameter is 145.55±8.64 nm. This result showed that, the addition of collagen can improve the morphology of PVA/chitosan nanofibers, where the characteristics of fibers made depending on the concentration of collagen added.


Keywords


Chitosan; collagen; PVA; nanofiber; electrospinning

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Sirajudin A, Rahmanisa S. Nanopartikel Perak sebagai Penatalaksanaan Penyakit Infeksi Saluran Kemih. Majority. 2016 Oct; 5(4):1-5.

Suryawan A, Kinho J. Diversity Species of Eboni (Diospyros Spp.) Tulabolo-Pinoguresort, Boganinani Wartabone National Park, North Sulawesi. Widyariset. 2012 Aug; 15 (2): 117-126.

Hulupi M, Haryadi. Sintesis dan Karakterisasi Serat Nano Polivinil Alkohol yang Diikat Silang dengan Glutaraldehid untuk Aplikasi Pembalut Luka. Chimica et Natura Acta. 2018 Dec; 6 (3): 101-105.

Herdiawan H, Juliandri, Nasir M, editors. Pembuatan Dan Karakterisasi Co-PVDF Nanofiber Komposit Menggunakan Metode Elektrospinning. Prosiding Seminar Nasional Sains dan Teknologi Nuklir PTNBR – BATAN Bandung; 2013 July 4; Bandung, Indonesia. P.110-116.

Supriyanto A, Murni M.L, Marlina F, Pangga D. Uji Biokompatibilitas Nanofiber Komposit Kitosan/PVA Sebagai Pembalut Luka. Jurnal Kependidikan Fisika. 2018 June; 6 (1): 37-42.

Yulina R, Winiati W, Bonadies I, Laurienzo P, Dalpogetto G. Optimasi Kondisi Larutan dan Parameter Proses Pemintalan Elektrik pada Sintesis Serat Nano Kitosan-PEO. Arena Tekstil. 2018 July; 33 (1): 1-10.

Nuryantini A.Y, Munir M.M, Suciati T, Khairurrijal, editors. Pembuatan Serat Nano PVA/Kitosan dengan Penambahan Sodium Tripolifosfat (STTP) sebagai Agen Ikatan Sambung Silang. Seminar Nasional Material 2013; 2013 Feb 16; Bandung, Indonesia. P. 70-73.

Hartomo B.T, Firdaus, F.G. Pemanfaatan Biomaterial Kitosan dalam Bidang Bedah Mulut. Jurnal Kedokteran Gigi Universitas Baiturrahmah. 2019 June; 6 (1): 63-70.

Octavian A. Kajian Sifat Fisik-Mekanik dan Antibakteri Plastik Kitosan Termodifikasi Kolagen Limbah Sisik Ikan Kakap Merah [Thesis]. Semarang: Universitas Negeri Semarang; 2015.

Ratnawati A, Izak D.R, Supardi A. Sintesis dan Karakterisasi Kolagen dari Teripang-Kitosan sebagai Aplikasi Pembalut Luka. Journal of Physics and Application. 2013 Apr; 1 (2): 12-24.

Sudirman, Karo A.K, Sukaryo S.G, Adistiana K.D, Dahlan K. Synthesis of Nanofiber from Poly Vinyl Alcohol (PVA)-Collagen using Electrospinning Methods. Jurnal Kimia Terapan Indonesia. 2020 Apr; 21 (2): 55-65.

Pavia D.L, Lampman G.M, Kriz G.S, Vyvyan, J.R. Introduction To Spectroscopy. United States: Brooks/Cole, Cengage Learning; 2001.

Windari, N.N.P., Rupiasih, N.N., Sumadiyasa, M. Studi Membran Kitosan dari Kulit Lobster Bambu Sebagai Membran Filtrasi. Buletin Fisika. 2016 Feb; 17 (1): 1-6.

Alchaddad, M., Siadi, K., Supartono. Transesterifikasi Etil P-Metoksisinamat Hasil Isolasi Rimpang Kencur dengan Vitamin C Terkatalis Lipase. Indonesian Journal of Chemical Science. 2015 Aug; 4 (2): 84-88.

Suptijah, P., Indriani, D., Wardoyo, S.E. Isolasi dan Karakterisasi Kolagen Dari Kulit Ikan Patin (Pangasius sp.). Jurnal Sains Natural Universitas Nusa Bangsa. 2018 Jan; 8 (1): 8-23.

Dachriyanus. Analisis Struktur Senyawa Organik Secara Spektroskopi. Padang: Lembaga Pengembangan Teknologi Informasi dan Komunikasi (LPTIK) Universitas Andalas; 2012.

Wivanius, N. and Budianto, E. Sintesis dan Karakterisasi Hidrogel Super absorben Kitosan Poli(N-Vinil kaprolaktam) (PNVCL) Dengan Metode Full IPN (Interpenetrating Polymer Network). Pharm Sci Res. 2015 Dec; 2 (3): 152-168.

Gonggo, S.T., Diah, A.W.M., Lateene, R. Pengaruh Kaolin Terhadap Membran Blend Kitosan Poli Vinil Alkohol-Litium Sebagai Membran Elektrolit Untuk Aplikasi Baterai Ion Litium. Jurnal Akademi Kimia. 2017 Feb; 6 (1): 55-64.

Suryati, Azhari, Pasaribu, D.L. Pembuatan Biokomposit Kitosan/ Alginat/Kolagen untuk Aplikasi Pembalut Luka [Thesis]. Medan: Universitas Sumatera Utara; 2021.

Sari, I.W., Nita, W.O., Rupiasih, N.N., Sumadiyasa, M. Pengaruh Paparan Radiasi UVC Terhadap Struktur dan Swelling Membran Kitosan Yang Terpapar Medan Magnet. Buletin Fisika. 2019 July; 20 (2): 35-40.

Hidayati, A., and Cahyono, E. Matrix Composition Effect on the Characteristics of Isopulegol Nanofibers Fabrication by Electrospinning Method. Indonesian Journal of Chemical Science. 2016 May; 10 (1): 49-58.

Zubair, M. To Increase Data Analysing Power You Must Know Frequency Distribution (Stat-04) [Internet]. 2021 [updated 2021 Aug 10; cited 2022 Oct 30]. Available from: https://towardsdatascience.com/to-increase-data-analysing-power-you-must-know-frequency-distribution-afa438c3e7a4.

Darmawan, M., Syamdidi, Yennie, Y., Wibowo, S. Karakteristik Serat Nano Komposit Kitosan-Polivinil Alkohol (PVA) dari Cangkang Rajungan melalui Proses Electrospinning. JPB Kelautan dan Perikanan. 2016 Nov; 11 (2): 213-222.




DOI: https://doi.org/10.18860/neu.v15i2.17665

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