ESTIMATION OF CEPHALOSPORINS (CEFTRIAXONE, CEFTAZIDIME) ANTIBIOTICS AS PURE AND PHARMACEUTIC FORMS BY COLOR PRODUCED REACTION IN UV-VIS SPECTROPHOTOMETIC TECHNIQUE

Mohauman Mohammad Majeed Al-Rufaie, Zahraa Yosif Motaweq

Abstract


A new simple, accurate and low cost-effective UV-VIS spectrophotometric methods has been developed for the analysis of chosen cephalosporins (ceftriaxone, ceftazidime) in bulk samples and pharmaceutical dosage forms by using a specific  color-generated reaction. This method involves the Schiff 's base formation reaction between selected cephalosporins with alcoholic 5-sulfo salicylaldehyde reagent 5SSA with heating to establish a yellow colored product displaying λmax at 426 nm as well as 412 nm sequentially. These methods applied to the Beer’s law through the range of concentration was  (4 - 60 µg.ml-1), (5 -50µg ml-1) respectively for two drugs. The analysis results for these studies  have been tested statistically by the studies of recovery. These methods were successfully applied to the determination of the cephalosporins in bulk form and in Pharmaceuticals additionally it was shown that these methods have equivalent accuracy with the common method(BP)estimation procedures for the studied  drugs. The interferences from frequently taken excipients were  no influence on the estimation process .The study could discover a good  as an employment cost-influence and speed method which that possibility used for making quality control for  these cephalosporin antibiotics.

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References


M. E. Abdel-Hamid: FSQ spectrophotometric and HPLC analysis of some cephalosporins in the presence of their alkali induced degradation products,Il Farmaco,1998;53 (2),132–138.

O. A. Adegoke, and O. E. Umoh: A new approach to the spectrophotometric determination of metronidazole and tinidazole using p-dimethyl amino benzaldehydes, Acta Pharm., 2009;59(4), 407–419.

J. N. Delgado and W.A. Remers: Wilson and Griswold's Textbook of Organic Medicinal and Pharmaceutical Chemistry, tenth ed. Lippincott Williams & Wilkins, New York, 2004,653.

C. Dollery: Therapeutic Drugs, vol. 1, third ed. Churchill Livingstone, Edinburgh, 1999,321.

A. F. M. El-Walily, A. A. Gazy, S. F. Belal, and E. F. Khamis: Selective spectrofluorometric determination of phenolic b-lactam antibiotics through the formation of their coumarin derivatives, J. Pharm. Biomed. Anal., 1999, 20 (4), 643–653.

K. Kelani, L. I. Bebawy, and L. Abdel-Fattah:Stability-indicating spectrophotometric and densitometric methods for determination of some cephalosporins, J.AOAC Int., 1998, 81 (2), 386–393.

G.H. Frederick: Penicillin, cephalosporin, and other - lactam antibiotics. Goodman and Gillman’s. The pharmacological basis of therapeutics. 11th ed. New York: McGraw Hills Medical Publishing Division,2006,321.

Gladwin and Mark :Clinical Microbiology Made Ridiculously Simple 4th ed.. Miami, FL: Med Master, Inc,2007, 67.

J.F.Graumlich:Lactam antibiotics .Modern pharmacology with clinical applications. 6th edition. Philadelphia: Lippincott Williams & Wilkins, 2003,554.

Martindale-The Complete Drug Reference, 33rd ed., Pharmaceutical Press, London, 2002,174.

The British Pharmacopoeia, Her Majesty’s Stationary Office, London, 2009,1163,1169,8255.

US Pharmacopoeia XXIIth Rev., US Pharmacopoeia Convention, 2009,329,521.

F.A. Aly, M. M. Hefnawy, and F. Belal: A selective spectrofluorometric method for the determination of some a-amino cephalosporins in formulations and biological fluids, Anal. Lett.,1996,29 (1), 117–130.

D. Agbaba, S. Eric, K. Karljikovic-Rajic, S. Vladimirov, and D. ZivanovStakic: Spectrophotometric determination of certain cephalosporins using ferrihydroxamate method, Spectroscope. Lett.,1997,30 (2), 309–319.

J.H. Yang, Q.L. Ma, X. Wu, L. M. Sun, and X.H. Cao:A new luminescence spectrometry for the determination of some balsamic antibiotics, Anal. Lett.,1999, 32 (3), 471–480.

M. M. Hefnawy Y. El-Shabrawy, and F. Belal: Spectrofluorometric determination of alpha-amino cephalosporins in biological fluids and pharmaceutical preparations, J. Pharm. Biomed. Anal.,1999, 21 (4), 703–707.

H. Yao, Y. Tang, Y. Li, and Y. Sun: Flow injection chemiluminescence determination of cephalosporin antibiotics by their enhancing effects on luminal-potassium periodate system, Anal. Lett.,2003 36 (14), 2975–2983.

Y.Li and J. Lu: Chemiluminescence flow injection analysis of B lactam antibiotics using the luminal-permanganate reaction, Luminescence,2006, 21 (4), 251–255.

C. Thongpoon, B. Liawruangrath, S. Liawruangrath, R.A. Wheatley, and A. Townshend: Flow injection chemiluminescence determination of cefadroxil using potassium permanganate and formaldehyde system, J. Pharm. Biomed. Anal., 2006,42 (2), 277– 282.

V.M. Shinde, and C.V. Shabadi, C.V: Simultaneous determination of cefadroxil and cefalexin from capsules by reversed phase HPLC, Indian Drugs,1997,34, 399–402.

V.M. Shinde, and C.V. Shabadi, C.V: Simultaneous determination of cephazolin and cefotaxime from injections by reversed phase HPLC, Indian J. Pharma. Sci.,1998, 60 (5), 313–315.

G.Misztal: Determination of cefotaxime and cefotriaxone in pharmaceuticals by HPLC,Pharmazie,1998,53,723–724.

W.R. La Course and C.O. Dasenbrock: Pulsed electrochemical detection of sulfur containing antibiotics following high performance liquid chromatography, J. Pharm. Biomed. Anal., 1999,19 (1–2), 239–252.

N. A. El-Maali, A. M. M. Ali, and M.A. Ghandour: Electrochemical reduction and oxidation of two cephalosporin antibiotics: ceftriaxone (rocephin) and cefoperazone (cefobid), Electro analysis (N.Y.),1993, 5 (7), 599–604.

L. N. C. Rodrigues, M. B. V. Zanoni, and A. G. Fogg: Indirect polarographic and cathodic-stripping voltammetric determination of cefaclor as an alkaline degradation product, J. Pharm. Biomed. Anal.,1999, 21 (3), 497–505.

V. S. Ferreira, M.V.B. Zanoni, and A.G. Fogg: Cathodic stripping voltammetric determination of ceftazidime with reactive accumulation at a poly-L-lysine modified hanging mercury drop electrode, Anal. Chim. Acta,1999, 384 (2), 159–166.

S. A. Ozkan, N. Erk, B. Uslu, N, Yilmaz, and I. Biryol: Study on electro oxidation of cefadroxil monohydrate and its determination by differential pulse voltammetry, J. Pharm. Biomed. Anal.,2000, 23 (2–3), 263–273.

K. R. Patel, V. D. Patel, K. P. Patel and V. G. Patel:Development and Validation of Spectrophotometric Method for Determination of Ceftriaxone Sodium in Pharmaceutical Dosage Forms, Der Pharma Chemica,2010,2(5),255–259.

M. M. Al-Rufaie, A.N.Al-Sharefy, K.H.Kathem: a New spectrophotometric method for the determination chlorpromazine hydrochloride in pharmaceutical preparations by using oxidative coupling reaction. Inter. J. of Uni. Pharmacy and Bio Sciences, 2013,2(4), 184-189.

M. M. Al-Rufaie: a New spectrophotometric method for the determination of Sulfamethoxazole drug, W.J.of pharmacy and pharmaceutical sciences,2016,5(3),172-180.

A. A. Jawad and K.H.Kathem: spectrophotometric determination of metoclopramide hydrochloride in bulk and pharmaceutical preparations by the diazotization-coupling reaction, Inter.J.of Pharmacy and Pharmaceutical Sciences,2013.5(3),294-298.

F. M. A. Rind, M. G. H. Laghari, A. H. Memon, U.R. Mughal, F. Almani, N. Memon, M. Y. Khuhawar, and M. L. Maheshwari: Spectrophotometric Determination of Ceftriaxone Using 4-Dimethylaminobenzaldehyde, Pak.J.Anal. Environ. Chem.,2008,9(2),42–48.

M. M. Al-Rufaie: Modern kinetic spectrophotometric procedure for estimation of furosemide drug as bulk form and in pharmaceuticals preparations, Curr. Issues Pharm. Med. Sci.2016,29(4),184–189.

B. Hiremath, B.H. Mathada, and M. unjayaswam: Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms, Acta Pharm.,2008,58, 275–285.

M. I. H. Helaleh, E. S.M. Abu-Namch, and R. M. A. Q. Jamhour: Direct titration and indirect spectrophotometric determination of selected cephalosporins, Acta poloniae pharmaceutica,1998,55(2),93–97.

A. A. Ramadan, H. Mandil, and M. Dahhan: UV-VIS Spectrophotometric study for determination of cefixime in pure form and in pharmaceuticals through complexation with Cu(II) using acetate–NaOH buffer in water: methanol, International J. of Pharmacy and Phar. Sci.,2013,5(1),428–433.

A. J. Abdulghani, and S. K. Mohuee: Synthesis of gold nanoparticles using ceftriaxone sodium as a reducing and stabilizing agent, Iraqi Journal of Science,2015,56(3C),2425–-2438.




DOI: https://doi.org/10.18860/jip.v3i2.6098

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