Roihatul Mutiah, Yolanda Cahyaning Sukma, Dewi Sinta Megawati, Rahmi Annisa


The root of Calotropis gigantea (thistle) is an Indonesian herb to treat cancer based on empirical and scientific evidences. The study aims to find out the infuence of ethanol extract of Calotropis gigantea root in inhibiting cancer cell growth of Mus musculus fibrosarcoma in vivo and to find out metabolite compound in the Calotropis gigantea root extract. The effect of cancer cell growth inhibition was tested on Mus musculus inducted with 7.12-dimetilbenz (α) antrasena (DMBA) and dose treatment of 50, 100 and 150 mg/Kg body weight. The metabolomic analysis on the root employs UPLC-QToF-MS/MS as a positive ESI ion source, the movement phase of water/acid mixture is 99.9/0.1 [v/v] and acetonitrile/formic acid was 99.9/0.1 [v/v] with gradient elution system and stationary phase of C18. The chromatogram was analyzed using Masslynx 4.1. The component identification was based on the m/z ratio measured in Masslynx and m/z counted in chemdraw. The result of the study showed that the Calotropis gigantea root extract with the dose of 50, 100 dan 150 mg/Kg body weight were able to increase the weight of fibrosarcoma mice and have a significant influence on caspase-3 expression with cell apoptosis index 24.3 %; 13.3 % and 12.3 % respectively. The result of metabolomic analysis showed 14 compounds found in the root extract. There are two major compounds: 4-Chlorobenzenethiol with the area 32.51% and N-[1-(Adamantan-1-yl) ethyl]-2-(1-piperidinyl)-4-quinazolinamine with the area 37.20%. They are indicated responsible for the anticancer activity of Calotropis gigantea root extract.

 Keywords: Calotropis gigantea, caspase-3, fibrosarcoma, metabolomic, UPLC-QToF-MS/MS

Full Text:



Hassan I., Konchok D., Abdul S., Parvaiz A. 2013. Sunscreens and Antioxidant Photo-Protective Measures: an Update. Post Graduate Department of Dermatology, STD & Leprosy, Govt. Medical College, Srinagar, University of Kasmir, J & K, India

Kalaiselvi M, Kalaivani K. 2012. Phytochemcial analysis and anti-lipid peroxidative effect of Jasminum sambac (L.) Ait oleaceae. Pharmacologyonline; 1: 38-43.

Damogalad, Viondy, Hosea Jaya Edy, Hamidah Sri Supriati. 2013. Formulasi Krim Tabir Surya Ekstrak Kulit Nanas (Ananas comosus L Merr) Dan Uji In Vitro Nilai Sun Protecting Factor (SPF). Pharmacon Jurnal Ilmiah Farmasi – Unsrat ;2(02): 39-44.

Nikumbh, VK, Sevankar, SG, and Patil, MP 2013. Formulation Development, In Vitro Evaluation of Microemulsion-based gel Loaded With Ketoprofen. Informa Healthcare, USA.

Departemen Kesehatan Republik Indonesia. 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Edisi I. Direktorat Jenderal Pengawasan Obat dan Makanan. Direktorat Pengawasan Obat Tradisional. Jakarta.

Direktorat Jendral Pengawasan Obat dan Makanan. 2000. Metode Analisis PPOM. Departemen Kesehatan RI. Jakarta.

Winarno, FG. 2002. Kimia Pangan dan Gizi. Gramedia. Jakarta.

Hendradi, E., Purwamti, T., Suryanto, AA 2013. Characterization of Preparations and Diclofenac Sodium Release Test with Microemulsion System in HPMC Gel Base. PharmaScientia. Vol 1, No. 2.

Yulianti, Erlina., Adeltrudis Adelsa., And Alicia Putri. Determination of Value SPF (Sun Protection Factor) of 70% Ethanol Extract Temu Mangga (Curcuma mango) and 70% Ethanol Extract Cream Temu Mangga (Curcuma mango) Vitro In Method Using spectrophotometry. Essay. Faculty of Medicine and Health Sciences. Brawijaya University. Poor.

Murahata, RI and Aronson, PM 1994. The Relationship Between Solution pH and Clinical Irritancy for Carboxylic Acid-based Personal Washing Products. J. Soc. Cosmet. Chem. 45: 239-246.

Fauzy, A. Effect of Fish Oil Concentration on Curcumin Penetration in Microemulsion Preparations. 2012. Thesis. Faculty of Mathematics and Natural Sciences, University of Indonesia. Depok

Utami, Suci Syafitri. 2012. In Vitro Formulation and Penetration Test of Nanoemulsion Preparation, Nanoemulsion Gel, and Curcumin Gel. Essay. Faculty of Mathematics and Natural Sciences, University of Indonesia. Depok

Martin, A., Swarbrick, J., Cammarata, A., 1993. Physical Pharmacy. UI-Press. Jakarta. Thing 1077-1090.



  • There are currently no refbacks.

Copyright (c) 2019 Roihatul Mutiah, Tias Pramesti Griana, Weka Sidha Bhagawan, Burhan Ma'arif, Maratus Soliha, Elok Kamilah Hayati, Hajar Sugihantoro, Arief Suryadinata, Christyaji Indradmojo, Retno Susilowati

© 2023 Journal of Islamic Pharmacy