Characteristics of Goat Ovarium Granulosa Cells With Extract Leaves ‎‎(Schleichera oleosa) at Different Passage Levels in Vitro

Lil Hanifah, Roihatul Muti’ah

Abstract


S. oleosa belongs to the Sapindaceae family and has phytochemicals phenolic acid so that it has enormous benefits in the process antioxidant. The presence of antioxidant activity in cells can affect the defense of the cell protein membrane, so that the ability of cells to perform division is optimal. In vitro this is mostly done by adding hormones to cell culture media or natural compounds to increase cell proliferation. Every population of cell that has undergone division during the proliferation process to confluent is an indication that the cell is aging. This study was an experimental study was designed using a completely randomized design (CRD) with 5 treatments and 5 replications as follows: K0: granulose cells and culture media without treatment (0 µl), P1: granulose cells and culture media with 1% kesambi leaf extract. (30 µl), P2: granulose cells and culture media given kesambi leaf extract 1.5% (45 µl), P3: granulose cells and culture media given kesambi leaf extract 2% (60 µl) and P4: granulosa cells and The culture media was given 2.5% (75 µl) to the leaves extract., the characteristics of goat ovarian granulosa cells by giving kesambi (Scheichera oleosa) leaf extract at different passages were at the level of confluence showing the highest value in passage I, namely treatment P1. Meanwhile, the level of viability shows the highest value in section I, namely the P4 treatment

Keywords


Granulose Cells; Kesambi Leaf Extract;‎ Passage;‎ In Vitro

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References


Anuragi, J. L., & Mishra, R. (2017). ‎Ethnomedicinal study of Schleichera ‎oleosa among the tribals of Satna ‎‎(M.P.). 3(3), 3.‎

Arundina, I., & Suardita, K. (2014). Efek ‎pegagan (Centella asiatica L) terhadap ‎proliferasi mesenchymal stem cell ‎‎(Effect of pegagan (Centella asiatica L) ‎to mesenchymal stem cell ‎proliferation). Journal of ‎Dentomaxillofacial Science, 13(1), 43. ‎https://doi.org/10.15562/jdmfs.v13i1.386‎

Bhatia, H., Kaur, J., Nandi, S., Gurnani, V., ‎Chowdhury, A., Reddy, P. H., ‎Vashishtha, A., & Rathi, B. (2013). A ‎review on Schleichera oleosa: ‎Pharmacological and environmental ‎aspects. Journal of Pharmacy Research, ‎‎6(1), 224–229. ‎https://doi.org/10.1016/j.jopr.2012.11.003‎

Budiono, D. (2018). Karakterisasi Rat ‎Embryonic Fibroblast (Ref) Pasca-‎Kriopreservasi Lambat Pada Tingkat ‎Pasase Yang Berbeda. Institut ‎Pertanian Bogor.‎

CABI. (2020). Schleichera oleosa (Macassar oil ‎tree). Https://Www.Cabi.Org/ ‎Isc/Datasheet/49004 #toidentity.‎

Freshney, ian. (2016). Culture of Animal Cells: ‎A Manual of Basic Technique and ‎Specialized Applications, 7th Edition.‎

Hati, R. (2016). Pengaruh Ekstrak Ethanol Daun ‎Sisik Naga (Pyrrosia pilosellaides) ‎Terhadap Tingkat Kematangan Folikel ‎Ovarium Kambing (Capra Aegagrus ‎Hircus) Secara In Vitro. uin maulana ‎malik ibrahim malang.‎

Holil, K., & Griana, T. P. (2020). Analisis ‎Fitokimia dan Aktivitas Antioksidan ‎Ekstrak Daun Kesambi (Schleichera ‎oleosa) Metode DPPH. 5.‎

Kalanjati, woro. (2006). Perbedaan ‎Konfluensitas dan Viabilitas Sel Kultur ‎Primer Sel Fibroblas Dari Jaringan Daun ‎Telingan Rusa Bawean (Axis kuhlii) ‎Pada Medium TCM 199 dan MEM. ‎Universitas Airlangga.‎

Kwist, K., Bridges, W. C., & Burg, K. J. L. ‎‎(2016). The effect of cell passage ‎number on osteogenic and adipogenic ‎characteristics of D1 cells. ‎Cytotechnology, 68(4), 1661–1667. ‎https://doi.org/10.1007/s10616-015-9883-‎‎8‎

Moreau, R. A., Whitaker, B. D., & Hicks, K. B. ‎‎(2002). Phytosterols, phytostanols, and ‎their conjugates in foods: Structural ‎diversity, quantitative analysis, and ‎health-promoting uses. Progress in ‎Lipid Research, 41(6), 457–500. ‎https://doi.org/10.1016/S0163-‎‎7827(02)00006-1‎

Panche, A. N., Diwan, A. D., & Chandra, S. R. ‎‎(2016). Flavonoids: An overview. ‎Journal of Nutritional Science, 5, e47. ‎https://doi.org/10.1017/jns.2016.41‎

Parwata, I. (2016). Antioksidan Bahan Ajar. ‎Pasca Sarjana Universitas Udayana.‎

Pogorelov, A., & Katkov, igor. (2007). ‎Influence of Exposure to vitrification ‎solutions on 2-cell mouse embryos: I. ‎Intracellular potassium and sodium ‎content. PubMed, 26(6), 403–408.‎

Rahmawati, M. (2014). Potensi Ekstrak Daun ‎Widuri (Calotropis gigantea) Sebagai ‎Obat Antikanker Fibrosarkoma. uin ‎maliki press.‎

Sitorus, M. S. (2013). Imunoekspresi Ki-67 Pada ‎Tumor Payudara Tikus Wistar Yang ‎Diinokulasi Tumor Terinduksi Benzo(Α) ‎Pyrene Dan Diberikan Ekstrak Daun ‎Sirsak. Universitas Sumatra Utara.‎

subandi, imam. (2018). Profil Protein Ovarium ‎Tikus Putih (Rattus norvegicus) Betina ‎Setelah Pemberian Ekstrak Etanol Daun ‎Sisik Naga. uin maulana malik ibrahim ‎malang.‎

Sumiati, T., Gardenia, L., & Sunarto, A. (2009). ‎PEMBUATAN KULTUR SEL PRIMER ‎DARI SIRIP EKOR IKAN MAS (Cyprinus ‎carpio). Jurnal Riset Akuakultur, 4(1), ‎‎107. ‎https://doi.org/10.15578/jra.4.1.2009.107‎‎-116‎

Waseh, laneng. (2016). Pengaruh Lama ‎Paparan Murottal Surat Al-Fatihah ‎Terhadap Proliferasi Sel Granulosa ‎Kambing (Capra Aegagrus Hircus) ‎Secara In Vitro. uin maulana malik ‎ibrahim malang




DOI: https://doi.org/10.18860/elha.v8i1.11419

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