OPTIMIZATION AND QUERCETIN RELEASE TEST OF MORINGA LEAF EXTRACT (MORINGA OLEIFERA) IN GEL-MICROEMULSION PREPARATION

Weka Sidha Bhagawan, Ria Ramadhani Dwi Atmaja, Sri Nur Atiqah

Abstract


Quercetin is a flavonoid compound of the flavonol group that has various biological activities for health. Moringa leaf is one of the plants rich in quercetin which is about 384.61 mg / 100 g. Quercetin is practically insoluble (4.5 μg / mL). To increase the solubility and release of quercetin microemulsion may be used as Drug Delivery Systems formulated with gels for transdermal use.

The aim of this research is to obtain optimum combination formulation of surfactant (tween 80): cosurfactant (propylene glycol) to form optimal microemulsion system, to find out the characteristics of the preparation and to find out the rate of quercetin release of Moringa leaf extract (M. oliefera) in a gel-microemulsion preparation compared with a control gel (without microemulsion) using an HPMC (Hydroxypropyl methylcellulose) gel basis.

The result shows that optimal concentration tween 80 comparison : propylene glycol to form optimal microemulsion system up to 30%: 20 % with VCO (virgin coconut oil) oil phase 5%. The characteristics observed including organoleptic, pH, viscosity, and stability are showing optimal condition. The average of quercetin content in gel-microemulsion preparation was 2,22 ± 0,077 ppm and on control gel was 2.37 ± 0.042 ppm. Quercetin release test using Franz diffusion cell using cellophane membrane, saline phosphate buffer pH 7,4 ± 0,05 and temperature 37 ± 0,5 °C for 6 hours. The average quercetin release flux in the gel-microemulsion preparation was 9.436 ± 1.178 (μg/cm 2/min1/2) and the control gel was 5,816 ± 0.485 (μg/cm2/min1/2).Based on these results it can concluded that the microemulsion gel preparation gives significant difference of quercetin flux rate compared with gel preparation.

 

Keywords: Quercetin, Gel-Microemulsion, quercetin release, moringa leaf extract (Moringa oliefera)


Full Text:

PDF

References


Rahmat, H. 2009. Identifikasi Senyawa Flavonoid Pada Sayuran Indigenous Jawa Barat [skripsi]. Bogor: Program Teknologi Pertanian Institut Pertanian Bogor.

Gilley, A.D; Arca, H.C; Nichols, B.I.B dan Giraldo, L.I.M. 2017. Novel cellulose- based amorphous solid dispersion enhance quercetin solution concentrations in vitro. Elseiver. Vol, 157: 89-91.

Tjay, Tan Hoan dan Kirana Rahardja, 2007, Obat-Obat Penting Khasiat, Penggunaan dan Efek-Efek Sampingnya Edisi Keenam. Jakarta: PT. Elex Media Komputindo.

] Kakran, M; Sahoo, N.G; Lin, L dan Muller R.H. 2011. Comprasiaon Of Homogenization And Precipation Techniques For Production Of Quercetin Nanocyrstal. Cameca Jurnal: 2-9.

Syofyan; Lucida, H dan Bakhtiar, A. 2008. Peningkatan Kelarutan Kuersetin Melalui Pembentukan Kompeks Inklusi Dengan b-Siklodekstrin. Jurnal Sains Dan Teknologi Farmasi. Vol, 13, No. 2: 43 – 48.

Lawrence, M.J dan Gareth, D. R. 2000. Microemulsion Based Media As Novel Drug Delivery System. Elseiver Advence Druh Delivery Reviews.Vol, 45 (2000): 89-121.

Harwansh, R.K; Rahman, M.A dan Dangi, J.S. 2010 Microemulsion System For Transdermal Delivery Of Diclofenac Soium For Bioavailability Enhancement.Journal Of Pharmacy Research. Vol, 3(9): 2183-2185.

Hardianti, F. 2015. Pemanfaatan Aktivitas Antioksidan Ekstrak Daun Kelor (Moringa oliefera) Dalam Sediaan Hand and Body Cream [skripsi]. Jakarta: Program Studi Kimia UIN Syarif Hidayatullah.

Viyoch, J.2003. Development of o/w emulsion containing tamarind fruit pulp etxract. Naraseuan University Journal. Vol, 11 (3): 29-49.

Pathak, Y dan Thassu, D. 2009. Drug delivery nanoparticle formulation and characterization. New York: Informa Helathcare.

Rahmawanty, D; Anwar, E dan Bahtiar A. 2014. Formulasi Gel Menggunakan Serbuk Daging Ikan Haruan (Channa Striatus) Sebagai Penyembuh Luka. Media Farmasi. Vol, 11(1): 29-40.

Arikumalasari, J; Dewantara, I G.N.A dan Wijayanti, N.P.AD. 2013. Optimasi HPMC Sebagai Gelling Agent Dalam Formula Gel Ekstrak Kulit Buah Manggis (Garcinia mangostana L.). Naskah Publikasi: 145-148.

Handayani, D.W; Yusriada dan Hardani.R. 2016. Formulasi Mikroemulsi Ekstrak Terpurifikasi Daun Bayam Merah (Amaranthus TricolorL.) Sebagai Suplemen Antioksidan. Galenika Journal of Pharmacy Vol. 3 (1) : 1 – 9

Hendradi, Esti; Purwanti,T Dan Suryanto, Aryco Andy. 2012 Karakterisasi Sediaan Dan Uji Pelepasan Natrium Diklofenak Dengan Sistem Mikroemulsi Dalam Basis Gel HPC-M. Pharmascienta, Vol 1, No 2: 20-24.

Sinko, P.J. 2011. Farmasi Fisik dan Ilmu Farmasetika Edisi 5. Jakarta: EGC Kedokteran.

Garg, A.D; Aggarwal, S.G dan Sigla A.K. 2002. Spreading Of Semisolid Formulation. Pharmaceutical Technology. p: 84- 104.

Williams A.C dan barry B.W. 2004 Penetration Enhancer. Advanced Drug Deliv ery Review. Vol, 26(5): 02-16.

Harmi, Liza. 2014. Pembuatan Nanogingerol Dari Ekstrak Jahe (Zingiber Officinale Rosc) Menggunakan Homogenizer Dengan Kombinasi Inversi Komposisi dan Suhu. Naskah Publikasi Institut Pertanian Bogor, p: 30-39.




DOI: http://dx.doi.org/10.18860/jip.v2i2.4508

Refbacks

  • There are currently no refbacks.


Published by Department of Pharmacy, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Indonesia