Free-Radical Scavenging Activity (FRSA) of Secondary Metabolite Extracted from Indonesian Eucheuma spinosum

Abstract

I

Indonesia is the biggest country in the production of red seaweed of Eucheuma spinosum. The red seaweed has bioactive compounds that have a potential activity such as phenolic compounds as well as carrageenan and pigments. This paper reported phytochemical analysis of E. spinosum harvested by a local farmer in Sumenep Island, East Java and free-radical scavenging activity (FRSA) derived from 2,2’-diphenyl-1-picrylhdrazyl (DPPH) for several organic solvents. For extraction, dried powder is added with 5.0 mL of various solvents following by ultra-sonication assisted extraction for 30 minutes. The extract was separated by centrifugation for phytochemical analysis and radical scavenging evaluation. The prospecting of dichloromethane, ethyl acetate, and n-hexane extracts indicated the potency for radical scavengers. Alkaloids, terpenoids, and saponins were secondary metabolites that indicated the presence in the extracts. The best IC₅₀ value was presented by ethyl acetate extracts (384,86 ppm) with 38.78% for 50 ppm, while IC₅₀ values of n-hexane, methanolic, dicloromethane extracts were 410.12, 677.76 and 685.08 ppm, respectively.

Keywords: Eucheuma spinosum, phytochemical analysis, radical scavenging activity