Isolation and Identification of Cellulose Degrading Bacteria from Banana Peel Compost

Yendania Grevitara P., Badriyatur Rahma F., Hellen Septirangga P., Irma Dahlia Y., Endang Suarsini


Cellulolytic bacteria are bacteria that have the ability to hydrolyze cellulose complexes into smaller oligosaccharides and eventually become glucose. Glucose is used as a carbon and energy source for bacterial growth. This study was conducted to isolate the cellulose degrading bacteria from banana peel compost that produce cellulose enzymes based on the clear zone that visible around the colony. The cellulolytic activity was determined by the ability of bacteria to hydrolyze the Carboxymethyl Cellulose (CMC) substrate. Determination of cellulolytic activity is known based on cellulolytic index calculation, the diameter total minus the diameter of the colony and divided by the diameter of the colony. The result of five bacterial isolates was found but only one bacterium had the potential to be a cellulose degradation. Based on the Microbact Gram-Negative Identification System, the bacterium is Burkholderia cepacia. These bacteria have an important role in nature as decomposers of various complex compounds, such as cellulose, hemicellulose, lignin, and pectin.


Isolation, Identification, Cellulolytic bacteria, Banana peel compost

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Akita, Hironaga., Kimura, Zen., Yusoff Mohd., Nakashimma, Nobutaka dan Hoshino, Tamotsu. 2017. Identification and Characterization of Burkholderia multivorans CCA53, BMC Research Notes NCBI. (Online) (

Baharuddin, A.S., Razak, M.N.A., Hock, L.S., Ahmad M.N., Aziz, S.A., Rahman, N.A.A dan Shah, U.K.M. 2010. Isolation and Characterization of Thermophilic Cellulase-Producing Bacteria from Empty Fruit Bunches-Palm Oil Mill Effluent Compost, American Journal of Applied Sciences, 7: 56-62.

FAO. 2004. Fertilizer use by crop in Brazil. Land and Plant Nutrition Management Service. Land and Water Development Division. Food and Agriculture Organization of The United Nations. Rome. http://ftp.fao. org/agl/agll/docs/fertusebrazil.pdf 64 pp.

Gupta, Pratima, Samant, Kalpana, Sahu, Avinash. 2011. Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential. International Journal of Microbiology. Volume 2012.

Howard, R.L., Masoko, P. and Abotsi, E,. 2003. Enzyme activity of a Phanerochaete chryso-sporium cellobiohydrolase (CBHI. 1) expressed as a heterologous protein from Escherichia coli. African Journal of Biotechnology, 2(9). 296-300. Sept 2003.

Lamid, M., Nugroho, T.P., Chusniati, S. dan Rochiman, K. 2011. Eksplorasi bakteri selulolitik asal cairan rumen sapi potong sebagai bahan inokulum limbah pertanian. Jurnal Ilmiah Kedokteran Hewan 4(1): 37-42.

Ohara, Caroline M., 2005. Manual and Automated Instrumentation for Identification of Enterobacteriaceae and Other Aerobic Gram-Negative Bacilli. Clinical Microbiology Reviews, Jan 2005, p. 147-162.

Perez, J., J.M. Dorado, T. Rubia, J. Martinez. 2002. Biodegradation and Biological treatments of Cellulose, Hemicellulose and Lignin: An Overview. Int. Microbiol. 5, 53-63.

Sausa, Silvia., Ramos, G.C & Leitau, H. J. 2011. Burkolderia cepacia Complex: Emerging Multihost Pathogens Equipped with a Wide Range of Virulence Factors and Determiants. International Journal of Microbiology DOI: 10. 1155-2011-607575.

Schwarz, W. H., 2001. The Cellulosome and cellulose degradation by anaerobic bacteria. Appl Microbiol Biotechnol 56:634–649.

Van Antwerpen, T., R.S. Rutherford, and J.L. Vogel. 2002. Assessment of sugarcane endophytic bacteria and rhizospheric burkholderia species as antifungal agents. Proceeding of South African Sugar Technology Association 76:301-304.



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