The Effectiveness of Bay Leaf Extract (Eugenia polyantha) on the Stamina Enhancement of Male Mice (Mus musculus)

Dalilah Sofiah Izzati, Delima Engga Maretha

Abstract


Bay leaves are a common culinary spice used in Indonesian cuisine and are known to contain various chemical compounds including essential oils, flavonoids, tannins, antimicrobial compounds, and vitamin C. This study aims to investigate the effectiveness of bay leaf extract in enhancing the stamina of male mice. The research was conducted using a quantitative experimental method with a completely randomised design (CRD). A total of 20 male mice were used as samples and divided into five treatment groups, which included a control group (P0), a dosage of 3 mg/g body weight (P1), a dosage of 6 mg/g body weight (P2), a dosage of 12 mg/g body weight (P3), and a dosage of 18 mg/g body weight (P4), with each treatment repeated three times. The swimming test was employed to measure the duration of swimming in mice as an indicator of stamina. Data were analysed using ANOVA. The results indicated that the administration of bay leaf extract at various doses over a period of 7 days did not produce a significant (P=0,715) effect on the stamina enhancement of male mice. Therefore, bay leaf extract was not effective in improving the stamina of male mice at the tested dosages.

Keywords


Bay Leaf Extract, Stamina, Mice

Full Text:

PDF

References


1. Konda, M. R., Alluri, K. V., Janardhanan, P. K., Trimurtulu, G., & Sengupta, K. (2018). Combined extracts of Garcinia mangostana fruit rind and Cinnamomum tamala leaf supplementation enhances muscle strength and endurance in resistance trained males. Journal of the International Society of Sports Nutrition, 15(1), 1–14.

2. Yuliati, M. (2012). Uji Aktivitas Antimikroba Ekstrak Daun Salam (Syzygium polyanthum (Wight Walp.) Terhadap Beberapa Mikroba Patogen Secara KLT-Bioautografi. In עלון הנוטע (Vol. 66, Issue 2).

3. Agustina, R., Indrawati, D. T., & Masruhin, M. A. (2015). Aktivitas ekstrak daun salam. Laboratorium Penelitian Dan Pengembangan Farmaka Tropis Fakultas Farmasi Universitas Mulawarman, Samarinda, Kalimantan Timur, 120–123.

4. Sunia Widyantari, A. A. A. S. (2020). Formulasi Minuman Fungsional Terhadap Aktivitas Antioksidan. Widya Kesehatan, 2(1), 22–29.

5. Novira, P. P., & Febrina, E. (2019). Review Artikel: Tinjauan Aktivitas Farmakologi Ekstrak Daun Salam (Syzygium Polyanthum (Wight.) Walp). Jurnal Kesehatan Perintis (Perintis’s Health Journal), 16(1), 127–134.

6. Amyra, W. (2017). Pengaruh Ekstrak Rimpang Temulawak (Curcuma xanthorriza Roxb.) Terhadap Aktivitas Fisiologis Manusia Dengan Metode Harvard Step Test. (Undergraduate Thesis) Universitas Andalas, 564, 1–73.

7. Putriningtyas, N. D., Cahyati, W. H., & Rengga, W. D. (2021). Aktivitas Fisik Asupan Makanan Dan Kualitas Tubuh (1ed.). (N.Siyam, Ed.) Semarang: LPPM Universitas Negeri Semarang.

8. Elizabeth C Bryda. 2013. The Mighty Mice: The Impact of Rodents on Advances in Biomedical Research. The Journal of the Missouri State Medical Association Mo Med.110(3):207-211.

9. Akbar, R., Weriana, Siroj, R. A., & Afgani, M. W. (2023, Januari). Experimental Research Dalam Metodologi Pendidikan. Jurnal Ilmiah Wahana Pendidikan, 465-474.

10. Nazir, M. 1988. Research Methods. Jakarta: Ghalia Indonesia.

11. Aning Yulianingtyas and Bambang Kusmartono. 2016. Optimazition Of Solvent Volume and Maceration Time on Extraction of Flavnoids From Averrhoa Bilimbi Leaves. Teknik Kimia, 10, 58–64.

12. Windasari, P. P. (2020). Uji Efek Tonikum Ekstrak Etanol Daun Seledri (Apium graveolens L.) Terhadap Mencit Jantan (Balb/C). SKRIPSI, 21(1), 1–9.

13. Sani K, F., Yuliawati, Y., Herlina, H., & Yolandini, R. (2020). Uji efek tonikum ekstrak daun sawo manila (Manilkara zapota) pada mencit putih jantan (Mus musculus) dengan metode ketahanan renang. Riset Informasi Kesehatan, 9(1), 37.

14. Khairani, Elsa Yuniarti, R. S. (2018). Pengaruh Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) Terhadap Histologis Pankreas Mencit (Mus muculus L. Swiss Webster) Yang Diinduksi Sukrosa.

15. Muliani, I Nyoman Mangku Karmaya, K. T. (2014). Pemberian Kalsium Laktat dan Berenang Meningkatkan Osteoblast pada Epiphysis Tulang Radius Mencit Perimenopause. Jurnal Veteriner, 15(1), 39–45.

16. Yan, K., Gao, H., Liu, X., Zhao, Z., Gao, B., & Zhang, L. (2022). Establishment and identification of an animal model of long-term exercise-induced fatigue. Frontiers in Endocrinology, 13, 915937.

17. Anwar, K., Wathan, N., & Triyasmono, L. (2011). Efek Tonik Ekstrak Etanol Daun Pandan Wangi (Pandanus Amaryllifolius Roxb.) pada Mencit Jantan. Konferensi Nasional Sains Dan Aplikasinya (KNSA), June 2016, 381–385.

18. Loha, M., Mulu, A., Abay, S. M., Ergete, W., & Geleta, B. (2019). Acute and subacute toxicity of methanol extract of Syzygium guineense leaves on the histology of the liver and kidney and biochemical compositions of blood in rats. Evidence‐Based Complementary and Alternative Medicine, 2019(1), 5702159.

19. Ani, S., Indarto, D., & Cilmiaty, R. (2022). Subchronic effects of bay leaves (syzygium polyanthum) extract on body weight, physical changes and mortality in female wistar rats with low iron diet. Northwestern Medical Journal, 2(3), 138-144.

20. Frohlich, J. (2020, may 20). Rat and Mice. PMC PubMed Central, 345-367.

21. Nurman, S. W., & Rifki, M. S. (2015, Juni). Tinjauan Pemberian Sumplemen Makanan Terhadap Daya Tahan Otot Atlet Sepak Bola Poster FC Kecamatan Lembah Gumanti Kabupaten Solok. Jurnal Stamina, 2(2), 115-126,

22. Aditia, L., Badruzaman, Paramitha, S. T., & Jajat. (2018, Mei). Perbandingan Hasil Tes Vo2mak Atlet Renang Menggunakan Williams Swimming Beep Test dan Bleep Test. Jurnal Terapan Ilmu Keolahragaan, 3(1), 53-56.

23. Widodo, f. t., Susiyarti, & Prabandari, S. (2023). Kajian Efek Stimulan Minuman Kemasan Sachet Terhadap kelelahan Pada Mencit Putih Jantan (Mus musculu,L) Dengan Metode Swim Test. Jurnal Ilmiah Farmasi, 1-7.

24. Fajriyan Hakimi Lukman and Vivi. 2013. Anti–fatigue Test of Combination of Palm Sap and Sugar Cane Water Using The Swimming endurance method (Natatory exhaustion) on male mice. Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta. Pharmacy. 10(2): 1693–3591.

25. Rahayu, M Sunarti, S Silistiarini, D Prawiroatmodjo, S. 2006. Traditional Use of Medicinal Plants by Local Communities on Wawonii Island, Southeast Sulawesi. Departement of Biology FMIPA. UNS:Surakarta 7(3):245–250.




DOI: https://doi.org/10.18860/elha.v10i2.35128

Refbacks

  • There are currently no refbacks.


Copyright (c) 2025 El-Hayah:Jurnal Biologi

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

INDEXING OF El-Hayah :

----------------------------------------------------------------------------------

EDITORIAL OFFICE

Program Study of Biology
Faculty of Science and Technology
State Islamic University Maulana Malik Ibrahim, Malang, Indonesia
Jl. Gajayana No. 50 Malang 65144
Telp./Fax: (+62 341) 558933

e-mail: elhayah@uin-malang.ac.id

E-ISSN 2657-0726 | P-ISSN 2086-0064

----------------------------------------------------------------------------------


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.