Aktivitas Antibakteri Ekstrak Etanol Biji Keluak (Pangium Edule) Terhadap Bakteri Salmonella Typhi
Abstract
Background: Typhoid fever often occurs in various countries in the world and generally occurs in countries with low levels of cleanliness. Typhoid fever is a systemic disease caused by Salmonella typhi bacterial infection. The main therapy for typhoid fever is antibiotics. The challenge of using synthetic antibiotics nowadays is the emergence of side effects of resistance. One of the plants that can be used as an antibacterial is extract of keluak seed (Pangium edule). This study aims to determine the antibacterial activity of keluak seed (Pangium edule) antibacterial extract on the growth of Salmonella typhi. This research is a quantitative type of experimental research with true experimental post-test control design). Method: The method used in this study used a qualitative phytochemical test and dilution of the tube. This study uses 6 concentrations which are 5, 10, 15, 20, 25, and 30 mg/ml. Bacterial suspension was used as negative control and extract 100 mg/ml was used as positive control. Result: Based on the phytochemical test show that the seeds of the keluak seed (Pangium edule) contain alkaloids and terpenoids. The extract of keluak seed seed has a MIC at a concentration of 10 mg/ml and MKC 30 mg/ml. MKC data were analyzed by the KruskalWallis test (significance p = 0,000) with (coefficient correlation= -0,990) the relationship between the two variables is very strong. Conclusion: The extract keluak seed (Pangium edule) seeds can inhibit the growth of S. typhi bacteria.
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WHO, 2018. "Weekly epidemiological record". WHO 93, 153–172.
Depkes RI, 2013. "Sistematika Pedoman Pengendalian Penyakit Demam Tifoid". Direktorat Jendral Pengendalian Penyakit & Penyehatan Lingkungan, Jakarta.
Lailiyah, S.H., Athiroh, N., Santoso, H., 2018. Behavior Identification of Patients’ Post Typhoid Year 2016 in Subdistrict – District - Lowokwaru of Malang City 4, 7.
Alba, S., Bakker, M.I., Hatta, M., Scheelbeek, P.F.D., Dwiyanti, R., Usman, R., Sultan, A.R., Sabir, M., Tandirogang, N., Amir, M., Yasir, Y., Pastoor, R., van Beers, S., Smits, H.L., 2016. Risk Factors of Typhoid Infection in the Indonesian Archipelago. PLOS ONE 11, e0155286. https://doi.org/10.1371/journal.pone.0155286
Riedel, S., Morse, S.A., Mietzner, T., 2019. Medical Mikrobiologi Jawetz, Melnick, & Adelberg Ed. 28. McGraw-Hill Education, New York.
Utami, E.R., 2012. Antibiotika, Resistensi, dan Rasionalitas Terapi. SAINSTIS 1, 124–138.
Suswati, I., Juniarti, A., 2011. Sensitivitas Salmonella typhi terhadap Kloramfenikol dan Seftriakson di RSUD Dr. Soetomo Surabaya dan di RSUD Dr. Saiful Anwar Malang Tahun 2008-2009. J. Biomedika 3, 27–32.
Fitriyah, N., Wahuningsih, N., Kismanto, J., 2013. Obat Herbal Antibakteri Ala Tanaman Binahong. J. KesMaDaSka 116–122.
LIPI Press (Ed.), 2015. Kekinian keanekaragaman hayati Indonesia, 2014, Cetakan kedua. ed. LIPI Press, Menteng, Jakarta.
Sari, R., Suhartati, 2015. Pangi (Pangium Edule Reinw.) Sebagai Tanaman Serbaguna Dan Sumber Pangan. Info Tek. EBONI 12, 23–37.
Heruwati, E. sri, lsmaini, L., Mangunwardoyo, W., 2009. Antibactertal Test of Pangium (Pangium Edule Reinw) Extract Against The Growth of Fish Spoilage Bacteria. Researih Cent. Mar. Fish. Prod. Process. Biotechnol.-Petamburan 15, 65–73.
Mamuaja, C.F., Lumoindong, F., 2017. Aktivitas Antimikroba Ekstrak Biji Kluwek (Pangium Edule) sebagai Bahan Pengawet Alami Bakso Ikan Tuna 20, 10.
Mufti, N., Bahar, E., Arisanti, D., 2017. Uji Daya Hambat Ekstrak Daun Sawo terhadap Bakteri Escherichia coli secara In Vitro. J. Kesehat. Andalas 6, 289–294. https://doi.org/10.25077/jka.v6.i2.p289-294.2017
Ningrum, R., Purwanti, E., Sukarsono, S., 2017. Alkaloid Compound Identification of Rhodomyrtus tomentosa Stem as Biology Instructional Material for Senior High School X Grade. J. Pendidik. Biol. Indones. 2, 231–236. https://doi.org/10.22219/jpbi.v2i3.3863
Dewi, M.K., Ratnasari, E., Trimulyono, G., 2014. Aktivitas Antibakteri Ekstrak Daun Majapahit (Crescentia cujete) terhadap Pertumbuhan Bakteri Ralstonia solanacearum Penyebab Penyakit Layu. Lentera Bio 3, 51–57.
Kemenag, R. (Ed.), 2010. Tafsir ilmi, Cet. 1. ed. Lajnah Pentashihan Musahaf al-Qur’an, Badan Litbang & Diklat, Kementerian Agama RI, Jakarta.
Illing, I., Erfiana, Safitri, W., 2017. Uji Fitokimia Ekstrak Buah Dengen. J. Din. 8, 66–84.
Ergina, S.N., Pursitasari, I.D., 2014. Uji Kualitatif Senyawa Metabolit Sekunder pada Daun Palado (Agave angustifolia) yang Diekstraksi dengan Pelarut Air dan Etanol. J. Akad. Kim. 3, 165–172.
Afriani, N., Idiawati, N., Alimuddin, A.H., 2016. Skrining Fitokimiadan uji Toksisitas Ekstrak Akar Mentawa (Artocarpus anisophyllus) terhadap LARVA Artemia salina. JKK 5, 7.
Putri, H.D., Sumpono, Nurhamidah, 2018. Uji Aktivitas Asap Cair Cangkang Buah Karet (Hevea brassiliensis) dan Aplikasinya dalam Penghambatan Ketengikan Daging Sapi. J. Pendidik. Dan Ilmu Kim. 2, 97–105.
Habibi, A.I., Firmansyah, R.A., Setyawati, S.M., 2018. Skrining Fitokimia Ekstrak n-Heksan Korteks Batang Salam (Syzygium polyanthum). Indones. J. Chem. Sci. 4.
Anggara, Y., Teruna, H.Y., Jasril, 2014. Isolasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Ekstrak Metanol Dari Daging Biji Tumbuhan Pangium edule Reinw (Flacourtiaceae). Repos. FMIPA 1–6.
Warnasih, S., Hasanah, U., 2018. Ekstraksi Zat Warna dari Kluwek (Pangium edule reinw) Menggunakan Berbagai Pelarut. Ekologia, 1 18.
Listaty, H., Teruna, H.Y., Jasril, 2016. Uji Aktivitas Antibakteri dan Toksisitas Ekstrak n-Heksana Daging Biji Kluwak. Repos. FMIPA 7.
Manoppo, J.S.S., Sakul, E.H., Tengker, A.C., 2019. Potensi Bioinsektisida Dari Ekstrak Daun, Kulit Batang Dan Biji Tumbuhan Pangi (pangium edule reinw.) Dalam Meningkatkan Mortalitas Larva Crocidolomia Binotalis. J. Sains Dan Teknol. Univ. Negeri Manado 2, 9–19.
Jati, N.K., Prasetya, A.T., Mursiti, S., 2019. Isolasi, Identifikasi, dan Uji Aktivitas Antibakteri Senyawa Alkaloid pada Daun Pepaya. J. MIPA 6.
Faikha, N., 2018. Pengaruh Konsentrasi Etanol Sebagai Cairan Pengekstraksi Terhadap Aktivitas Antibakteri Dari Biji Pangi (Pangium edule Reinw) (Skripsi). Program Studi Farmasi Fakultas Farmasi Universitas Hasanuddin Makassar, Makasar.
Makagansa, C., Mamuaja, C.F., Mandey, L.C., 2015. The Antibacterial Activity of Pangi Kernel Extract (Pangium edule Reinw) towards Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli in Vitro. J Ilmu Dan Teknol. Pangan 3, 16–25.
Rahman, F.A., Haniastuti, T., Utami, T.W., 2017. Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668. Maj. Kedokt. Gigi Indones. 3, 1–7. https://doi.org/10.22146/majkedgiind.11325
Othman, L., Sleiman, A., Abdel-Massih, R.M., 2019. Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Front. Microbiol. 10, 911. https://doi.org/10.3389/fmicb.2019.00911
Cushnie, T.P.T., Cushnie, B., Lamb, A.J., 2014. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int. J. Antimicrob. Agents 44, 377–386. https://doi.org/10.1016/j.ijantimicag.2014.06.001
Harman-Ware, A.E., Sykes, R., Peter, G.F., Davis, M., 2016. Determination of Terpenoid Content in Pine by Organic Solvent Extraction and Fast-GC Analysis. Front. Energy Res. 4. https://doi.org/10.3389/fenrg.2016.00002
Ngajow, M., Abidjulu, J., Kamu, V.S., 2013. Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro. J. MIPA 2, 128. https://doi.org/10.35799/jm.2.2.2013.3121
Marisa, H., 2011. Isolasi Senyawa Antibakteri Dari Daun Jengkol (Pithecolobium lobatum Benth) dan Penentuan Nilai KHM-nya. J. Penelit. Sains 4.
Mahizan, N.A., Yang, S.-K., Moo, C.-L., Song, A.A.-L., Chong, C.-M., Chong, C.-W., Abushelaibi, A., Lim, S.-H.E., Lai, K.-S., 2019. Terpene Derivatives as a Potential Agent against Antimicrobial Resistance (AMR) Pathogens. Molecules 24, 2631. https://doi.org/10.3390/molecules24142631
Wahyuni, 2015. Uji Aktivitas Antibakteri Ekstrak Etanol Biji Picung (Pangium edule Reinw) Terhadap Bakteri Salmonella Typhi Atcc 13311 Secara Dilusi (Skripsi). UNIVERSITAS SETIA BUDI, Surakarta.
DOI: https://doi.org/10.18860/jim.v7i1.10546
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