Screening of Bacterial Isolates from Cow Manure with Potential as Antagonist Agents against Alternaria porri (Ellis) Cif.

Sherin Rizkina; Syukria Ikhsan Zam; Mokhamad Irfan; Irwan Taslapratama; Yusmar Mahmud; Siti Zulaiha; Oksana Oksana; Nida Wafiqah Nabila M. Solin

doi:10.18860/elha.v10i5.45721

Screening of Bacterial Isolates from Cow Manure with Potential as Antagonist Agents against Alternaria porri (Ellis) Cif.

Sherin Rizkina, Syukria Ikhsan Zam, Mokhamad Irfan, Irwan Taslapratama, Yusmar Mahmud, Siti Zulaiha, Oksana Oksana, Nida Wafiqah Nabila M. Solin

Abstract

Environmentally friendly control of Alternaria porri (Ellis) Cif. can be carried out using antagonistic agents such as bacteria. Exploration of bacterial sources as antagonistic agents needs to be continuously conducted, one of which is from cattle manure. This study aimed to obtain potential antagonistic bacteria against A. porri. Samples were collected using a purposive sampling technique. Bacterial isolation was performed using the spread plate method from dilutions of 10⁻⁶ to 10⁻⁸. Antagonistic activity was assessed using the dual culture method. This research obtained four isolates—PKa, PKb, PKc, and PKd—with cell counts ranging from 0.12 × 10⁹ to 1.83 × 10⁹ CFU g⁻¹. All isolates belonged to the Gram-positive group. Isolate PKa exhibited no catalase activity, whereas isolates PKb, PKc, and PKd showed catalase activity. PKa was identified as Clostridium, while PKb, PKc, and PKd were identified as Bacillus. All isolates demonstrated potential as antagonistic agents, with antagonistic activity ranging from 82% to 90%.

Keywords

Biological control; Bacillus; Clostridium; Purple blotch; Antifungal activity

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DOI: https://doi.org/10.18860/elha.v10i5.45721

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