Potensi Daun Genitri (Elaeoarpus Ganitrus) terhadap Profil Leukosit dan Histopatologi Lien Tikus Wistar Induksi Virus Influenza

Cicilia Novi Primiani, Pujiati Pujiati, Weka Sidha Bhagawan

Abstract


Background: Genitri (Elaeocarpus ganitrus) is a local plant, growing well in tropical forests. Genitri seeds are used as jewelry. Utilization of genitri in the health sector is still lacking. Objectif: To analyze the potential of genitri leaves on the leukocyte profile and splenic histopathology of Wistar rats. Methods: Experimental true approach, using experimental male wistar rats aged 5-6 months, weighing 280-360 grams, totaling 24 rats. Experimental animals were placed in group cages at 27oC, 12 hours of light, fed and watered ad libitum. Acclimatization was carried out 7 days before treatment. Treatment P0 (negative control), P1 induced influenza virus (Vaxigrip trivalent) 0.5 ml (positive control), P2 induced influenza virus 0.5 ml and 1 gram of genitri leaves. Induction of virus from intramuscular vaccine. Induction was carried out on day 0 and day 14. Genitri leaves were administered using the oral gavage method on the 3rd day for 29 days. On the 33rd day, blood sampling was carried out through the heart and surgery and spleen retrieval. Total leukocyte count using a hemocytometer. Histopathologically examined using hematoxylin and eosin (HE) staining. Results: The total leukocyte count on the 19th day, P0 4263.82 cells/mm3, P1 6314.46 cells/mm3, P2 8443.72 cells/mm3. Total leukocytes on day 33, P0 4241.66 cells/mm3, P1 4983.33 cells/mm3, P2 7520.83 cells/mm3. Diameter of germinal center spleen on day 19, P0 231 nm, P1 186 nm, P2 202 nm. Germinal center diameter on day 33, P0 239 nm, P1 191 nm, P2 242 nm. Tissue structure of the P1 spleen was congested and haemorrhagic, the structure of the spleen P2 had leukocyte infiltration, the marginal zone and germinal center were thickened. Conclusion: genitri leaves can increase the number of leukocytes and the diameter of the splenic germinal center of Wistar rats
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References


1. Freer G, Pistello M. Varicella-zoster virus infection: natural history, clinical manifestations, immunity and current and future vaccination strategies. New Microbiol. 2018;41(2):95–105.

2. Thomas SJ, Rothman AL, Srikiatkhaachorn A, Kalayanarooj S. Dengue virus infection: clinical manifestations and diagnosis. Hirsch MS, Hall KK, editors. Vol. 91, UpToDate. Waltham, Massachusetts, USA; 2018. 138–145 p.

3. Pritt BS, Christine M. Histopathology of viral infections of the lung. Semin Diagn Pathol. 2017;1–8.

4. Setiawan H, Wulandari SW, Fitriyani AN. Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar. J Vet. 2021;22(36):531–9.

5. Sharp TM, Keating MK, Shieh W, Bhatnagar J, Bollweg CB, Levine R, et al. Clinical characteristics, histopathology, and tissue immunolocalization of chikungunya virus antigen in fatal cases. Clin Infect Dis. 2021;73(2):e345–e354.

6. Yuliyanasari N. Global Burden Desease - Human Immunodeficiency Virus - Acquired Immune Deficiency Syndrome (HIV-AIDS). Qanun Med J Fac Med Muhammadiyah Surabaya. 2017;01(1):65–77.

7. Cook S, Conrad C, Fowlkes AL, Mohebbi MH. Assessing Google Flu Trends Performance in the United States during the 2009 Influenza Virus A (H1N1) Pandemic. PLoS One. 2011;6(8):1–8.

8. Br€ussow H, Br€ussow L. Clinical evidence that the pandemic from 1889 to 1891 commonly called the Russian fl u might have been an earlier coronavirus pandemic. Microb Biotechnol. 2021;0(0):1–11.

9. Fuaddah AT. Description of Self-Medication Behavior in Community of Subdistrict Purbalingga, District Purbalingga. J Kesehat Masy. 2015;3(1):610–9.

10. Mariella Delavega, Y., Pratiwi, L., & Rizkifani S. Analisis Tingkat Pengetahuan Mahasiswa Program Studi Farmasi terhadap Swamedikasi Influenza. J Syifa Sci Clin. 2022;4(2):263–74.

11. Bintang K, Zahro L, Komalasari L, Amir M, Erlangga MA, Kunaedi A. Review: Gambaran Swamedikasi terhadap Influenza pada Orang Dewasa dan Anak-Anak. Medimuh J Kesehat Muhammadiyah. 2023;4(1):19–26.

12. Jiang L, Deng L, Wu T. Chinese medicinal herbs for influenza (Review). Group CARI, editor. Sichuan, China: John Wiley & Sons, Ltd.; 2013. 1–61 p.

13. Siqueira CM, Homsani F, Couceiro N, Quaresma CH, Regina S, Passos L. Homeopathic medicines for prevention of influenza and acute respiratory tract infections in children: blind, randomized, placebo-controlled clinical trial. Homeopathy. 2015;(June 2014):1–7.

14. Chow EJ, Doyle JD, Uyeki TM. Influenza Virus-Related Critical Illness: Prevention, Diagnosis, Treatment. Crit Care. 2019;23(214):1–11.

15. Nypaver C, Dehlinger C, Carter C. Influenza and Influenza Vaccine: A Review. J Midwifery Women’s Heal. 2021;66(1):45–53.

16. Safitri A. Pentingnya Sistim Pembelajaran Online Di Masa Pendemi. 2021. p. 1–8.

17. Wądołowska L, Drywień M, Hamułka J, Socha P, Borawska M, Friedrich M, et al. Dietary Recommendations During The Covid-19 Pandemic. Statement of The Committee of Human Nutrition Science of The Polish Academy of Sciences. Rocz Panstw Zakl Hig. 2021;72(2):209–20.

18. Dores AR, Peixoto M, Castro M, Sá C, Carvalho IP, Martins A, et al. Knowledge and Beliefs about Herb / Supplement Consumption and Herb / Supplement – Drug Interactions among the General Population , including Healthcare Professionals and Decision System. Nutrients. 2023;15(2298):1–26.

19. Das S, Bordoloi R, Newar N. A Review on Immune Modulatory Effect of Some Traditional Medicinal Herbs. J Pharm Chem Biol Sci. 2014;2(1):33–42.

20. Gangal N, Nagle V, Pawar Y, Dasgupta S. Reconsidering Traditional Medicinal Plants to Combat Covid-19. AIJR. 2020;1–6.

21. Fennell CW, Lindsey KL, Mcgaw LJ, Sparg SG, Stafford GI, Elgorashi EE, et al. Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. J Ethnopharmacol. 2004;94:205–17.

22. Zhao X, Chen L, Lu J. A similarity-based method for prediction of drug side effects with heterogeneous information. Math Biosci. 2018;306:136–44.

23. Azis A. Kunyit (Curcuma domestica Val) sebagai Obat Antipiretik. J Ilmu Kedokt Dan Kesehat. 2019;6(2):116–20.

24. Kumari B, Srivastava A, Tiwari SK. Elaeocarpus spp.: A threatened power generating plant, its geographical distribution, propagation through in vivo condition and its medicinal aspects. Int J Fauna Biol Stud. 2018;5(2):27–31.

25. Baruah PS, Deka K, Lahkar L, Sarma B, Borthakur SK, Tanti B. Habitat distribution modelling and reinforcement of Elaeocarpus serratus L.-A threatened tree species of Assam, India for improvement of its conservation status. Acta Ecol Sin. 2018;1–8.

26. Zeno SSV, Nurjanah S, Muhaemin M. Karakteristik Kimia Minyak Ganitri (Elaeocoarpus ganitrus ROXB) Hasil Ekstraksi. J Penelit Pangan. 2016;1.

27. Hardainiyan S, Nandy BC, Kumar K. Elaeocarpus Ganitrus (Rudraksha): A Reservoir Plant with their Pharmacological Effects. Int J Pharm Sci Rev Res. 2015;34(1):55–64.

28. Joshi S, Gupta P, Kumar N, Rai N, Gautam P, Thapliyal A. A comprehensive report on therapeutic potential of Elaeocarpus ganitrus. Environ Conserv J. 2012;13(3):147–50.

29. Das P, Kar P, Hasnu S, Nath S, Tanti B. Phytochemical screening and antioxidant activity of Elaeocarpus serratus L . of Assam. J Pharmacogn Phytochem. 2017;6(4):866–9.

30. Ogundele AV, Das AM. Chemical Constituents from The Leaves of Elaeocarpus floribundus. Nat Prod Res. 2019;2–6.

31. Dalei J, Sahoo D. Evaluation of Antimicrobial Activity and Phytochemical Screening of Epicarp and Endocarp Parts of Elaeocarpus Ganitrus. Int J Pharma Bio Sci. 2016;7(2):265–9.

32. Jain PK, Sharma P, Joshi SC. Effect of Elaeocarpus ganitrus Roxb. on Htperlipidemia and Liver Function of Rabbit. Eur J Biomed Pharm Sci. 2016;3(10):326–33.

33. M AB, N SS, Lakshmi NS. Analysis of Therapeutic Potential and Computer Aided Virtual Screening of Elaeocarpus Ganitrus. Int J Sci Technol Eng. 2017;4(1):1–6.

34. Hardainiyan S, Nandy BC, Saxena R. Phytochemical investigation of fruit extract of Elaeocarpus ganitrus. Int J Pharm Pharm Sci. 2015;7(6):415–8.

35. Talukdar N, Dutta A, Chakraborty R, Das K. Screening of Phytochemicals, Antioxidant and Inhibitory Effect on Alpha-Amylase by Ethanolic Extract of Elaeocarpus ganitrus (BARK). Int J Pharm Sci Res. 2017;8(12):5270–5.

36. Jain PK, Sharma P, Joshi SC. Role of Elaeocarpus ganitrus Roxb. in Prevention of Coronary Artery Disease in Cholesterol fed Rabbits. Int J Pharm Sci. 2017;42(15):74–81.

37. Dadhich A, Rishi A, Sharma G, Chandra S. Phytochemicals of Elaeocarpus with Their Therapeutic Valu : A Review. Int J Pharma Bio Sci. 2013;

38. Jawla S, Rai DV. Pharmacognostic Studies on Rudraksh ( Elaeocarpus angustifolius Blume ) Fruit. 2016;10(6):382–7.

39. Kang SC, Park DW. Pharmaceutical Composition for Preventing and Treating Alpha Herpes Virus Infection, Containing, As Active Ingredient, Elaeocarpus Sylvestris Extract or Fraction Thereof. Pat Appl Publ. 2019;1:1–12.

40. Dubey GA. Effect of Extract of Rudraksha (Elaeocarpus Ganitrus) on Parkinson’s Disease and Depression. World J Pharm Res. 2018;7(12):937–47.

41. Hohakay JJ, Pontoh J, Yudistira A. Pengaruh Metode Pengeringan terhadap Kadar Flavonoid Daun Sesewanua (Clerodendron squamatum Vahl.). Pharmacon. 2019;8:748–57.

42. Salim AN, Sumardianto, Amalia U. Efektivitas Serbuk Simplisia Biji Pepaya sebagai Antibakteri pada Udang Putih (Penaeus merguensis) selama Penyimpanan Dingin. JPHPI. 2018;21(2):188–98.

43. Afrianti R, Ramadheni P, Irsanti PN. Uji Aktivitas Estrogenik Ekstrak Etanol Jintan Hitam (Nigella Sativa L.) Terhadap Perkembangan Uterus Tikus Putih Betina. sCIENTIA. 2017;7(1):49–55.

44. Carolina A. The Effect of Quadrivalent Influenza Vaccine on Lung Histopathological Changes of Male Wistar Rat (Rattus norvegicus). Dr Diss. 2019;

45. Spitalnik PF. Histology laboratory manual 2014-2015. Columbia: College of Physicians and Surgeons Columbia University; 2015. 0–104 p.

46. Anindya A, S. KP, Rantam FA, Rachmawati K, Nidom CA, Widiyatno T V., et al. Pengujian Vaksin Hepatitis B Fase Subkronis terhadap Berat Organ dan Diameter Pulpa Putih Limpa Tikus Putih (Rattus norvegicus). J Basic Med Vet. 2019;8(2):86–91.

47. Pratiwi AA. Uji Toksisitas Subkronis Oral Ekstrak Etanol Ubi Jalar Ungu (Ipomoea batatas L.) Kultivar Gunung Kawi terhadap Berat Organ dan Gambaran Histopatologi Limpa Tikus Wistar (Rattus norvegicus). Universitas Brawijaya 2018 p. 1–146.

48. Aldi Y, Dewi ON, Uthia R. Uji Imunomodulator dan Jumlah Sel Leukosit dari Ekstrak Daun Kemangi (Ocimum basilicum L.) pada Mencit Putih Jantan. Scientia. 2016;6(2):139–47.

49. Houwen B. Blood Film Preparation and Staining Procedures. Clin Lab Med. 2002;22(1):1–14.

50. Feldman AT, Wolfe D. Chapter 3. Tissue Processing and Hematoxylin and Eosin Staining. In: Methods in Molecular Biology. 2014. p. 31–43.

51. Xiang L, Hu X, Zhang J, She J, Li M, Zhou T, et al. Immunodepression induced by influenza A virus (H1N1) in lymphoid organs functions as a pathogenic mechanism. Clin Exp Pharmacol Physiol. 2020;47(10):1664–16731.

52. Jernigan DB, Lindstrom SL, Johnson JR, Miller JD, Hoelscher M, Humes R, et al. Detecting 2009 Pandemic Influenza A ( H1N1 ) Virus Infection : Availability of Diagnostic Testing Led to Rapid Pandemic Response. Supl Artic. 2011;52(Suppl 1):S36–43.

53. Mohan SS, McDermott BP, Cunha BA. The diagnostic and prognostic significance of relative lymphopenia in adult patients with influenza A. Am J Med. 2005;118(11):1307–1309.

54. Susilo A, Suwarto S, Rengganis I, Harimurti K. Peranan Gejala Klinis dan Pemeriksaan Darah Tepi dalam Like Illness Peranan Gejala Klinis dan Pemeriksaan Darah. J Penyakit Dalam Indones. 2014;1(2):96–107.

55. Lamichhane PP, Samarasinghe AE. Review Article The Role of Innate Leukocytes during Influenza Virus Infection. J Immunol Res. 2019;2019:1–17.

56. Nusa KC, Mantik MFJ. Hubungan Ratio Neutrofil dan Limfosit pada Penderita Penyakit Infeksi Virus Dengue. J e-Clinic. 2015;3(1):210–6.

57. Naumenko V, Turk M, Jenne CN, Kim S. Neutrophils in viral infection. Cell Tissue Res. 2018;1–12.

58. Nichols JE, Niles JA, Roberts NJ. Human Lymphocyte Apoptosis after Exposure to Influenza A Virus. J Virol. 2001;73(13):5921–9.

59. Nichols JE, Niles JA, Fleming EH, Roberts NJ. The role of cell surface expression of in fl uenza virus neuraminidase in induction of human lymphocyte apoptosis. Virology. 2019;534(June):80–6.

60. Mock DJ, Frampton MW, Nichols JE, Domurat FM, Signs DJ, Roberts NJ. Influenza Virus Infection of Human Lymphocytes Occurs in the Immune Cell Cluster of the Developing Antiviral Response. Viruses. 2018;10(420):1–21.

61. Roberts NJ, Horan PK. Expression of Viral Antigens after Infection of Human Lymphocytes, Monocytes, and Macrophages with Influenza Virus. Tha J Infect Dis. 1985;151(2):308–13.

62. Roberts NJ. Diverse and Unexpected Roles of Human Monocytes / Macrophages in the Immune Response to Influenza Virus. Viruses. 2020;12(379):1–8.

63. Cheng Y, Zhao H, Song P, Zhang Z, Chen J. Dynamic changes of lymphocyte counts in adult patients with severe pandemic H1N1 influenza A. J Infect Public Health. 2019;1–6.

64. Cole SL, Mcmichael AJ, Ho L, Cole SL, Dunning J, Kok WL, et al. M1-like monocytes are a major immunological determinant of severity in previously healthy adults with life-threatening influenza. JCI Insight. 2017;2(7):1–19.

65. Shen C, Ho T, Wang S, Hu Y, Tsai H, Chen S. The cellular immunophenotype expression of influenza A virus and influenza B virus infection in children. 2020. 1–33 p.

66. Kim JC, Kim HM, Kang YM, Ku KB, Park EH, Yum J, et al. Severe pathogenesis of influenza B virus in pregnant mice. Virology. 2014;448:74–81.

67. Singh B, Ishar MPS, Sharma A, Arora R, Arora S. Phytochemical and biological aspects of Rudraksha , the stony endocarp of Elaeocapus ganitrus ( Roxb .): a review. Isr J ofPlant Sci. 2015;(April):37–41.

68. Tripathy S, Mida A, Swain SR. Antimicrobial and hypoglycemic effects of Elaeocarpus Ganitrus Seed. Int J Community Sci Technol. 2016;2(1):70–6.

69. Yusuf MI, Jabbar A, Bafadal M, Malaka MH, Firdayanti, Sahidin I. Aktivitas Imunomodulator Ekstrak Etanol Spons Callyspongia sp. terhadap Fagositosis Makrofag pada Mencit Jantan. J Ilm Ibnu Sina. 2020;5(1):44–55.

70. Asif M. Chemistry and Antioxidant Activity of Plants Containing Some Phenolic Compounds. Chem Int. 2015;1(1):35–52.

71. Cecilia O, Olalekan O, Gbadura I. Tithonia diversifolia saponin-blood lipid interaction and its influence on immune system of normal wistar rats. Biomed Pharmacother. 2017;87:589–95.

72. Farias I, Araújo C, Sonego E, Luiz S, Luiz A, Alvarez-silva M, et al. Uncaria tomentosa Stimulates The Proliferation of Myeloid Progenitor Cells. J Ethnopharmacol. 2011;137(1):856–63.

73. Behl T, Kumar K, Brisc C, Rus M, Nistor-cseppento DC, Bustea C, et al. Exploring The Multifocal Role Of Phytochemicals As Immunomodulators. Biomed Pharmacother. 2021;133(October 2020):1–18.

74. Susilawati Y, Moektiwardoyo M, Halimah E, Wicaksono IA, Ramadhania ZM, Prastiwi SS. Aktivitas Imunomodulator Ekstrak Etanol Daun Pepaya (Carica papaya L.) dengan Metode Induksi Bakteri dan CBC-Diff. J Sains dan Kesehat. 2020;2(4):303–10.

75. Dao NT, Jang Y, Kim M, Nguyen HH, Pham DQ, Dang Q Le, et al. Chemical Constituents and Anti-influenza Viral Activity of the Leaves of Vietnamese Plant Elaeocarpus tonkinensis. Rec Nat Prod. 2019;13(1):71–80.

76. Ganeshpurkar A, Saluja A. In silico interaction of rutin with some immunomodulatory targets: a docking analysis. Indian J Biochem Biophys. 2018;55(April):88–94.

77. Cesta MF. Normal Structure, Function, and Histology of the Spleen. Toxicol Pathol. 2006;34:455–465.

78. Zhang Y, Garcia-ibanez L, Toellner K-M. Regulation of germinal center B-cell differentiation. Vol. 270, Immunological Reviews. John Wiley & Sons Ltd; 2016. 8–19 p.

79. Deng X, Wu F, Liu Z, Luo M, Li L, Ni Q, et al. The splenic toxicity of water soluble multi-walled carbon nanotubes in mice. Carbon N Y. 2009;47(6):1421–8.

80. Awaad A. Histopathological and Immunological Changes Induced by Magnetite Nanoparticles in the Spleen, Liver and Genital Tract of Mice Following Intravaginal Instillation. J Basic Appl Zool. 2015;(71):32–47.

81. Hassanen EI, Khalaf AA, Tohamy AF, Mohammed ER, Farroh KY. Toxicopathological and immunological studies on different concentrations of chitosan-coated silver nanoparticles in rats. Int J ofNanomedicine. 2019;14:4723–39.

82. Thalia Audina. PENGARUH PEMBERIAN EKSTRAK BUAH KIWI (ACTINIDIA DELICIOSA) TERHADAP BERAT DAN GAMBARAN MIKROSKOPIS ORGAN LIMPA TIKUS JANTAN STRAIN SPRAGUE DAWLEY YANG TELAH DIBERIKAN MONOSODIUM GLUTAMAT (MSG) SELAMA HARI. 2017 p. 1–85.

83. Chen Q, Liu Y, Lu A, Ni K, Xiang Z, Wen K, et al. Influenza virus infection exacerbates experimental autoimmune encephalomyelitis disease by promoting type I T cells infiltration into central nervous system. J Autoimmun. 2016;xxx:1–10.

84. Ueki H, Wang I, Fukuyama S, Katsura H, Jose T. In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs. PNAS. 2018;2–9.

85. Vogel AB, Haasbach E, Reiling SJ, Droebner K, Klingel K, Planz O. Highly Pathogenic Influenza Virus Infection of the Thymus Interferes with T Lymphocyte Development. J Immunol. 2010;(October).

86. Favaloro B, Allocati N, Graziano V, Ilio C Di, Laurenzi V De. Role of Apoptosis in disease. Aging (Albany NY). 2012;4(5):330–49.

87. Ludwig S, Pleschka S, Planz O, Wolff T. Ringing the alarm bells : signalling and apoptosis in influenza virus infected cells NA. Cell Microbiol. 2006;8(3):375–86.

88. Kearney JF. B cell subpopulations and secondary lymphoid organ architecture. In: Seminars in Immunology. 2008. p. 1–3.

89. Handono CD. Efek Imunostimulatori Beberapa Fraksi Teripang Lokal Phyllophorus sp terhadap Histologi Limpa Mencit (Mus musculus) yang Diinfeksi Mycobacterium tuberculosis. 2012.

90. Rousdy DW, Wardoyo ERP. HISTOLOGI LIMPA DAN HEMATOLOGI MENCIT YANG DIINFEKSI Escherichia coli SETELAH PEMBERIAN ASAM HUMAT GAMBUT KALIMANTAN. Bioteknol BIOSAINS Indones. 2018;5(2):168–76.

91. Alberts B, Johnson A, Lewis J, Al. E. Lymphocytes and the Cellular Basis of Adaptive Immunity. In: Molecular Biology of the Cell. 4th editio. New York: Garland Science; 2002. p. 132–42.

92. Prakoeswa FRS. Peranan Sel Limfosit Dalam Imunulogi: Artikel Review. J Sains dan Kesehat. 2020;2(4):525–37.

93. Zhang H, Gao J, Tang Y, Jin T, Tao J. Inflammasomes cross-talk with lymphocytes to connect the innate and adaptive immune response. J Adv Res. 2023;(xxxx).

94. Stebegg M, Kumar SD, Silva-cayetano A, Fonseca VR. Regulation of the Germinal Center Response. Front Immunol. 2018;9(October):1–13.

95. Jacob J, Kassir R, Kelsoe G. In Situ Studies of the Primary Immune Response to (4-hydroxy-3-nitrophenyl) acetyl. I. The Architecture and Dynamics of Responding Cell Populations. J Exp Med. 1991;173:1165–75.

96. Allen C, Okada T, Cyster J. Germinal-Center Organization and Cellular Dynamics. Immunity. 2007;27:190–202.

97. Manoharan AL, Thamburaj S, Muniyandi K, Jagadeesan G, Sathyanarayanan S, Nataraj G, et al. Antioxidant and antimicrobial investigations of Elaeocarpus tectorius (Lour.) Poir . fruits against urinary tract infection pathogens . Biocatal Agric Biotechnol. 2019;20.

98. Jain PK, Sharma P, Joshi SC. Anti-Atherosclerotic Activity of Elaeocarpus ganitrus Roxb. in Cholesterol-Fed Rabbits. Asian J Pharm Clin Res. 2017;10(12):10–4.

99. Miao L, Zhang Y, Yang X, Xiao J, Zhang H, Zhang Z, et al. Colored light-quality selective plastic films affect anthocyanin content, enzyme activities, and the expression of flavonoid genes in strawberry (Fragaria × ananassa) fruit. Food Chem. 2016;207:93–100.

100. Ramiro E, Franch A, Castellote C, Andres-Lacueva C, Izquierdo-Pulido M, Castell M. Effect of Theobroma cacao flavonoids on immune activation of a lymphoid cell line. Br J Nutr. 2005;93:859–66.

101. Pandey R, Maurya R, Singh G, Sathiamoorthy B, Naik S. Immunosuppressive properties of flavonoids isolated from Boerhaavia diffusa Linn. Int Immunopharmacol. 2005;5(6567):541–53.

102. Djati MS, Dwijayanti DR, Rifa’i M. Herbal Supplement Formula of Elephantopus scaber and Sauropus androgynus Promotes IL-2 Cytokine Production of CD4 + T Cells in Pregnant Mice with Typhoid Fever. Open Life Sci. 2016;11:211–9.

103. Mace TA, King SA, Ameen Z, Elnaggar O, Young G, Riedl KM, et al. Bioactive compounds or metabolites from black raspberries modulate T lymphocyte proliferation, myeloid cell differentiation and Jak/STAT signaling. Cancer Immunol Immunother. 2014;63(9):889–900.

104. Piekarska J, Szczypka M, Kucharska AZ, Gorczykowski M. Effects of iridoid-anthocyanin extract of Cornus mas L. on hematological parameters, population and proliferation of lymphocytes during experimental infection of mice with Trichinella spiralis. Exp Parasitol. 2018;1–27.




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